Sciencell™MycoplasmaPCRDetectionKitisa16SrRNA-basedPCRassayconsistingof2×PCRmastermix(whichincludesPCRbuffer,DNApolymeraseanddNTPs),mycoplasmaprimerset,andapositivecontrol,whichisnoninfectiousgenomicDNA(gDNA)ofM.fermentans.

Mycoplasma,thesmallestandself-replicatingprokaryotes,arecommoncontaminantsofprimarycellsandcelllines.Mycoplasmacontaminationcanaffectcellgrowth,morphologyandmetabolism.Mycoplasmaareabletopassthrough0.2µmfiltersduetotheirsmallsize.FurThermore,unlikebacteriaandfungi,mycoplasmacontaminationisresistanttocommonlyusedantibiotics,andcannotbeidentifiedvisually.Duetothedifficultiesindetectingmycoplasma,cellsshouldberegularlyscreenedtocontrolmycoplasmacontamination.ConventionalscreeningmethodsincludemicroBIOLOGicalculture,fluorescentDNAstaining,andbiochemicaldetectionmethods,andaretypicallytimeconsumingandlacksensitivity.Asaresult,polymerasechainreaction(PCR)basedmethodshavebeendevelopedasaquickandconvenientdetectionoflowlevelmycoplasmainfection,whileprovidinghighsensitivityandspecificity.

Alignmentstudiesofmycoplasma16SrRNAsequencesrevealedtheexistenceofhighlyconservedsequences.PrimerstargetingthesesequencesallowforthespecificamplificationofthemycoplasmaDNAfragment.ScienCell™MycoplasmaPCRDetectionKitisa16SrRNA-basedPCRassayconsistingof2×PCRmastermix(whichincludesPCRbuffer,DNApolymeraseanddNTPs),mycoplasmaprimerset,andapositivecontrol,whichisnoninfectiousgenomicDNA(gDNA)of M.fermentans.Thegenus-specificprimersetrecognizesthefivetypicalcontaminatingmycoplasmaspecies(i.e.,M.hyorhinis,M.arginini,M.orale,M.fermentans,andA.laidlawi),whichaccountfor98%ofcontamination,aswellasmembersofthegeneraUreaplasma,Spiroplasma,andAcholeplasma,whichaccountfortheremaining2%ofcontamination.EukaryoticandbacterialDNAisnotamplifiedbythiskit.Thesizeoftheexpectedbandis280bp.

Specifications:

CatalogNo.	8208
CountryofManufacture	UnitedStates
ProductCode	MYCO
Size/Quantity	100tests
ProductUse	Thisassaykitisusedtodetectmycoplasmainvitro.Itisforresearchuseonly.Notforuseinanimals,humans,ordiagnosticprocedures.
Storage	-20°C
ShippingInfo	Dryice.

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