ThisassaykitisusedtoevaluateLDHactivityinvitro.Itisforresearchuseonly.Notforuseinanimals,humans,ordiagnosticprocedures.
Lactatedehydrogenase(LDH),whichisasolublecytosolicenzymepresentinmosteukaryoticcells,releasesintoculturemediumuponcelldeathduetodamageofplasmamembrane.TheincreaseoftheLDHactivityinculturesupernatantisproportionaltothenumberoflysedcells.SciencellLDHCytotoxicityAssaykitprovidesacolorimetricmethodtomeasureLDHactivityusingreactioncocktailscontaininglactate,NAD+,diaphroseandINT.LDHcatalysesthereductionofNAD+toNADHinthepresenceofL-lactate,whiletheformationofNADHcanbemeasuredinacoupledreactioninwhichthetetrazoliumsaltINTisreducedtoaredformazanproduct.Theamountofthehighlycoloredandsolubleformazancanbemeasuredat490nmspectrophotometrically.
Specifications:
CatalogNo. | 8078 |
CountryofManufacture | UnitedStates |
ProductCode | LDH |
Size/Quantity | 500tests |
ProductUse | ThisassaykitisusedtoevaluateLDHactivityinvitro.Itisforresearchuseonly.Notforuseinanimals,humans,ordiagnosticprocedures. |
Storage | -20°C |
ShippingInfo | Dryice. |