Producedfromhumanfibroblastsmaintainedinserum-freemediaandthenpurifiedbiochemically.ThisissuppliedsterileinCAPSsalinebuffer.

Caution:Ifhandledimproperly,somecomponentsofthisproductmaypresentahealthhazard.Takeappropriateprecautionswhenhandlingthisproduct,includingthewearingofprotectiveclothingandeyewear.Disposeofproperly.

Fibronectinisamulti-domainglycoproteincomposedofanarrayofmultiplerepeatedmodularstructures.Cellularfibronectin,anadhesionglycoproteinoftheextracellularmatrix,existsasadimerwithamolecularmassof~550kDa. Thisstructureiscomposedoftwoheterodimers,theAchainandtheBchaincontainingthetypeIIIconnectingsegmentregion.Cellularfibronectindiffersfromplasmafibronectinbythepresenceofadditionalpolypeptidesegmentsanditscapacitytospecificallyregulatemorphologyandgrowthofattachedcellsincludingstemcells[1,2,3,4].Multipledomainsoffibronectinshowbindingaffinitiesforcollagen,fibrin,heparin,andspecificcellmembranereceptorssuchasintegrins.Fibronectinmatrixassemblyisessentialfornormaldevelopmentandcontributestothegenerationoftumormetastases.

Sciencell^TMHumanCellularFibronectin(HCF)isproducedfromhumanfibroblastsmaintainedinserum-freemediaandthenpurifiedbiochemically.HCFissuppliedsterileinCAPSsalinebuffer. 

ProductSpecification

Quantity:        0.1mg

Concentration:           0.5mg/ml

Storagebuffer: CAPSsalinebuffer,pH11.

Specifications:

CatalogNo.	8488
CountryofManufacture	UnitedStates
ProductCode	HCF
Size/Quantity	100μg
ProductUse	THESEPRODUCTSAREFORRESEARCHUSEONLY.Notapprovedforhumanorveterinaryuse,forapplicationtohumansoranimals,orforuseinclinicalorinvitroprocedures.
Storage	Itisrecommendedtostoretheproductassingleusealiquotsat-80°C.Thawingshouldbedoneslowlyat2-8°Cwithnoagitation.Materialthatfailstodissolvecanberemovedbycentrifugation.Avoidrepeatingfreeze/thawcycles.
ShippingInfo	Dryice.
References	1.YamadaKMandAkiyamaSK(1984).InMethodsforpreparationofmedia,supplementsandsubstrataforserum-freeanimalcellculture.pp.215-30.AlanR.Liss,Inc.,NewYork.2.AkiyamaSetal.(1985).FibronectinandfibronectinfragmentsinExtracellularMatrix:APracticalApproach,(NewYork),p.183.3.PottsJRandCampbellID(1994).FibronectinStructureandAssembly.Curr.CellBio,6:648-655.4.SinghP,SchwarzbauerJE(2012).Fibronectinandstemcelldifferentiation-lessonsfromchondrogenesis.JCellSci,15:3703-12.

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