Bovinehypothalamusextract(BHE)containsnumerousgrowthfactorsandhormonesthatpromoteendothelial[1],epithelial[2,3],andhybridomacellproliferation[4],especiallyinlowserumandserum-freemedia.
Thehypothalamusisacriticalbrainregionlinkingthenervousandendocrinesystemsthroughthepituitarygland.Thehypothalamusregulatesvitalhomeostaticprocesses,includingmetabolism,bodilytemperature,reproductivebehaviorsandfoodandwaterintake.Bovinehypothalamusextract(BHE)containsnumerousgrowthfactorsandhormonesthatpromoteendothelial[1],epithelial[2,3],andhybridomacellproliferation[4],especiallyinlowserumandserum-freemedia.

Specifications:

CatalogNo.	0613
CountryofManufacture	UnitedStates
ProductCode	BHE-5
Size/Quantity	10mg/ml;5x1ml
ProductUse	Recommendedconcentrationrange:5-100ug/ml.Thefinalworkingconcentration,however,shouldbeoptimizedforindividualcelltypeandcultureconditions.Note:Colormayvarybetweenlotsandlipoproteinprecipitatesmaybepresent.ThisdoesnotaffecttheBIOLOGicalactivity.BHEisforresearchuseonly.Itisnotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures.
Storage	StoreBHEbetween-20°Cand-80°Cuntilreadyforuse.Aliquottoavoidrepeatedfreeze-thawcycles
ShippingInfo	Dryice.
References	[1]MaciagT,CerundoloJ,IlsleyS,KelleyPR,ForandR.(1979)"Anendothelialcellgrowthfactorfrombovinehypothalamus:Identificationandpartialcharacterization".Proc.Natl.Acad.Sci.USA76:5674-5678.[2]GilchrestBA,CalhounJK,MaciagT.(1982)"Attachmentandgrowthofhumankeratinovytesinaserum-freeenvironment".JournalofCellularPhysiology112:197-206.[3]MaciagT,NemoreRE,WeinsteinR,GilchrestBA.(1981)"Anendocrineapproachtothecontrolofepidermalgrowth:serum-freecultivationofhumankeratinocytes".Science211:1452-1454.[4]PintusC,RansomJH,EvansCH.(1983)"Endothelialcellgrowthsupplement:acellcloningfactorthatpromotesthegrowthofmonoclonalantibodyproducinghybridomacells".JImmunolMethods61:195-200.Caution:Ifhandledimproperly,somecomponentsofthisproductmaypresentahealthhazard.Takeappropriateprecautionswhenhandlingthisproduct,includingthewearingofprotectiveclothingandeyewear.Disposeofproperly

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