PURO-1isforresearchuseonly.Itisnotapprovedforhumanoranimaluse,orforapplicationinclinicalorinvitrodiagnosticprocedures.
PuromycinisanaminonucleosideantibioticisolatedfromStreptomycesalboniger.Puromycinterminatesproteinsynthesisattheleveloftranslationinprokaryoticandeukaryoticcellsandisactiveagainstgram-positivebacteriaandweaklyactiveagainstgram-negativebacteria.ResistancetopuromycinisconferredbythepuromycinN-acetyl-transferase(pac)gene[1,2].Exogenousexpressionofthepacgeneiscommonlyusedinconjunctionwithpuromycintogeneratestablecelllines.Mostworkingconcentrationsrangefrom1-10μg/ml;however,theoptimalconcentrationshouldbedeterminedforindividualcelltypesandcultureconditions.Inmostcases,puromycinkills99%ofnon-resistantcellswithin48hours.

Specifications:

CatalogNo.	0553
CountryofManufacture	UnitedStates
ProductCode	PURO-1
Size/Quantity	10mg/ml;1ml
ProductUse	PURO-1isforresearchuseonly.Itisnotapprovedforhumanoranimaluse,orforapplicationinclinicalorinvitrodiagnosticprocedures.
Storage	Storeat-20°C;avoidfreeze-thawcycles.
ShippingInfo	Dryice.
References	[1]VaraJ,Perez-GonzalezJA,JimenezA.(1985)"BiosynthesisofpuromycinbyStreptomycesalboniger:characterizationofpuromycinN-acetyltransferase".Biochemistry24:8074-8081.[2]LacalleRA,PulidoD,VaraJ,ZalacainM,JimenezA.(1989)"MolecularanalysisofthepacgeneencodingapuromycinN-acetyltransferasefromStreptomycesalboniger".Gene79:375-380.

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