EachprimersetincludedinHMSC-C-qPCRkitarriveslyophilizedina2mLvial.Allprimersaredesignedandtestedunderthesameparameters:(i)anoptimalannealingtemperatureof65°C(with2mMMg2+,andnoDMSO);(ii)recognitionofallknowntargetgenetranscriptvariants;and(iii)specificamplificationofonlyoneamplicon. 

Humanmesenchymalstemcells(MSCs)areapopulationofmultipotentcellsthatcanbedifferentiatedintomultiplelineage-specificcells,whichcanformbone,fat,cartilage,muscleandtendon.Theprocessofcartilageformationfromcondensedmesenchymetissueisknownaschondrogenesis.SciencellhascreatedaconvenientqPCRkitfortheassessmentofhumanmesenchymalstemcellchondrogenesis.SOX9andCOL2A1qPCRprimersincludedinthekitallowforthedetectionandquantificationofearly-to-mid-andlate-stageofhumanMSCchondrogenesis,respectively¹.

Note:allgenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).

FourqPCRcontrolsareincludedinthiskittoverifysuccessfulreversetranscriptionofmessengerRNA(mRNA)tocomplementaryDNA(CDNA),revealthepresenceofgenomicDNA(gDNA)contaminationincDNAsamples,anddetectqPCRinhibitorcontamination.GoodqualitycDNAisacriticalcomponentforsuccessfulgeneexpressionanalysis.

EachprimersetincludedinHMSC-C-qPCRkitarriveslyophilizedina2mLvial.Allprimersaredesignedandtestedunderthesameparameters:(i)anoptimalannealingtemperatureof65°C(with2mMMg2+,andnoDMSO);(ii)recognitionofallknowntargetgenetranscriptvariants;and(iii)specificamplificationofonlyoneamplicon.EachprimersethasbeenvalidatedbyqPCRbymeltcurveanalysisandgelelectrophoresis.

Specifications:

CatalogNo.	8328
CountryofManufacture	UnitedStates
ProductCode	HMSC-C-qPCR
Size/Quantity	50reactions
ProductUse	HMSC-CPCRkitisforresearchuseonly.Notforuseinanimals,humans,ordiagnosticprocedures.
Storage	Storeat-20°Cuponreceipt.Avoidrepeatedfreezethawcyclesbymakingsixaliquotsat110μLeach.
ShippingInfo	Dryice.
References	[1]Sekiya,I.,Vuoristo,J.T.,Larson,B.L.,&Prockop,D.J.(2002).Invitrocartilageformationbyhumanadultstemcellsfrombonemarrowstromadefinesthesequenceofcellularandmoleculareventsduringchondrogenesis.PNAS,99(7),4397-4402.

Sciencell-试剂种类ScienCell研究实验室提供完整的高质量细胞培养试剂。我们还提供多种细胞转染试剂，以及您的细胞所需的任何细胞外基质。无论您需要USDA认证的胎牛血清，胰蛋白酶/ EDTA还是无酶解离溶液，我们都可以提供经过充分测试和保证的成分，使您的细胞发挥最大潜能。猫。没有。码描述0423GSN0.2％明胶溶液，100毫升0823国家航空航天局100X非必需氨基酸，100毫升86883D-CMK3D胶原蛋白I矩阵试剂盒88083D GSP3D凝胶染色准备套件0503P / S抗生素溶液5毫升0533阿姆斯抗生素/抗真菌溶液，50毫升0523AMS抗真菌溶液，50毫升0943天文天冬酰胺0603BHE-1牛下丘脑提取物（BHE-1）0613BHE-5牛下丘脑提取物（BHE-5）0763BPE-NZ牛脑垂体提取物–新西兰0753BPE-NZ牛脑垂体提取物–新西兰0713BPE牛脑垂体提取物，100毫克0703BPE牛垂体提取物，25毫克8248BPF牛血浆纤连蛋白8538BPV牛血浆玻连蛋白0600中央电视台细胞培养级水，500毫升0133CFM细胞冷冻培养基，50 ml0143SF-CFM不含血清的细胞冻结培养基，50毫升8188CCCSCK胶原蛋白I细胞培养表面涂层试剂盒MB6908DPDRIDualPrep DNA / RNA分离试剂盒0303DPBSDulbecco磷酸盐缓冲盐水，500毫升0903结束内源性凝集素0123经济合作发展组织无酶细胞解离液，100 ml0923EpiFEpiFectagen0973EpiFEpiFectagen II0010胎牛血清胎牛血清，10毫升0025胎牛血清胎牛血清25毫升0500胎牛血清胎牛血清500毫升0913纤维蛋白纤维凝集素