Ouradipogenesisdifferentiationmediumhasbeenspecificallydevelopedandoptimizedforinvitromesenchymalstemcelladipogenesisstudy.Generally,withthismedium,weobtain>35%matureadipocytesfromhumanbonemarrow,>50%fromratbonemarrow,and>60%fromhumanmesenchymalstemcellisolatedfromadiposetissue.Efficiencyofadipogenicdifferentiationdependsonthequanlityofthemesenchymalstemcells.MesenchymalstemcellAdipogenicDifferentiationMedium(MADM)isasterile,liquidmediumwhichcontainsessentialandnon-essentialaminoacids,vitamins,organicandinorganiccompounds,hormones,growthfactors,traceminerals.ThemediumisHEPESandbicarbonatebufferedandhasapHof7.4whenequilibratedinanincubatorwithanatmosphereof5%CO2/95%air.ComponentsMADMconsistsof500mlofbasalmedium,25mloffetalbovineserum(FBS,Cat.No.0025),5mlofmesenchymalstemcelladipogenicdifferentiationsupplement(MADS,Cat.No.7542),5mlofpenicillin/streptomycinsolution(P/S,Cat.No.0503).Note:MADSmaybeslightlyhazy;Hazinessdoesnotaffectcellcultureanddifferentiationefficiency.
Specifications:
CatalogNo. | 7541 |
CountryofManufacture | UnitedStates |
ProductCode | MADM |
Size/Quantity | 500ml |
ProductUse | MADMisforresearchuseonly.Itisnotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures. |
Storage | Storethebasalmediumat4°C,FBS,MADSandP/Ssolutionat-20°C.Protectfromlight. |
ShippingInfo | DryIce |