Macrophagesarecellsdifferentiatedfromcirculatingbonemarrow-derivedmonocytes.Inthebonemarrowandsubsequestlyinthebloodandtissues,monocytesundergoacascadeofmaturationthatculminateinmorphologicallyandfunctionallymaturetissuemacrophages[1].MacrophagesfunctiontoremovalcellulardebrisandinvADIngpathogens.Theyphagocytizeinvadingmicroorganisms,andscavengedead,damagedcellsandcellulardebris.MacrophagescanbeidentifiedbythespecificexpressionofseveralcellsurfaceproteinsincludingCD14,CD11b,F4/80(mice)/EMR1(human),MAC-1/MAC-3,andCD68byflowcytometryorimmunohistochemicalstaining[2].
RMa-bmfromScienCellResearchLaboratoriesareisolatedfromadultCD®IGSratbonemarrow.RMa-bmareharvestedatP0anddeliveredfrozen.Eachvialcontains>1x10^6cellsin1mlvolume.RMa-bmarecharacterizedbyimmunofluorescencewithantibodyspecifictoF4/80.RMa-bmarenegativeformycoplasma,bacteria,yeast,andfungi.RMa-bmareguaranteedtofurthercultureundertheconditionsprovidedbySciencellResearchLaboratories;however,RMa-bmarenotrecommendedforexpandingorlong-termculturessincethecellsdonotproliferateinregularculture.
RecommendedMedium
ItisrecommendedtouseMacrophageMedium(MaM,Cat.#1921)fortheculturingofRMa-bminvitro.
Specifications:
CatalogNo. | R1920 |
CountryofManufacture | UnitedStates |
ProductCode | RMa-bm |
Size/Quantity | 1x10^6cells/vial |
ProductUse | RMa-bmareforresearchuseonly.Theyarenotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures. |
Storage | Transfercellsdirectlyandimmediatelyfromdryicetoliquidnitrogenuponreceivingandkeepthecellsinliquidnitrogenuntilcellcultureisneededforexperiments. |
ShippingInfo | Dryice. |
References | [1].MartinClineandMargaretSumner(1972)BoneMarrowMacrophagePrecursors.I.SomeFunctionalCharacteristicsoftheEarlyCellsoftheMouseMacrophageSeriesBlood40(1)62-69.[2].SiamonGordonandPhilipTaylor(2005)Monocyteandmacrophageheterogeneity.NatureReviewsImmunology5(12)953-964. |