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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
alpha-TubulinAntibodyProperties Description Anti-alpha-Tubulin(RABBIT)Antibody-600-401-880 Host Rabbit TargetSpecies Human KnownCrossReactivity human,mouse,rat.chicken,bovine Clonality Polyclonal Application ELISA:1:90,000 IFMicroscopy:1:500-1:2,000 WesternBlot:1:1,000-1:5,000 Immunohistochemistry:1:500-1:2,000 OtherDilution:UserOptimized PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format IgG Label Unconjugated Concentration 1.1mg/mlbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 Preservative 0.01%(w/v)SodiumAzide StABIlizer None GeneName TUBA1B RelevantLinks NCBI-17986283 UniProtKB-P68363 |
alpha-TubulinAntibodyDescription
Background
TubulinLoADIngControlAntibodyrecognizesmicrotubuleswhichareinvolvedinawidevarietyofcellularactivitiesrangingfrommitosisandtransporteventstocellmovementandthemaintenanceofcellshape.Tubulinitselfisaglobularproteinconsistingoftwopolypeptides(alphaandbetatubulin).Alphaandbetatubulindimersareassembledto13protofilamentsthatformamicrotubuleof22-nmdiameter.TyrosineligaseaddsaC-terminaltyrosinetomonomericalphatubulin.AssembledmicrotubulescanagainbedetyrosinatedbyaCytoskeleton-associatedcarboxypeptidase.DetyrosinatedalphatubulinisreferredtoasGlu-tubulin.Anotherpost-translationalmodificationofdetyrosinatedalphatubulinisC-terminalpolyglutamylation,whichischaracteristicofmicrotubulesinneuronalcellsandthemitoticspindle. Thisantibodymakesanexcellentloadingcontrol.Anti-Alpha-tubulinantibodyisidealforinvestigatorsinvolvedincellcycleproteinresearch.
Synonyms
rabbitanti-alpha-TubulinAntibody,rabbitanti-a-tubulinantibody,Tubulinalpha-1Bchain,Tubulinalpha-ubiquitouschain,Alpha-tubulinubiquitous,TubulinK-alpha-1,TUBA1B,tubulinloadingcontrol,Alpha-tubulin,Tubulinalpha-1A,TUBA1A,TUBA3,LIS3
Immunogen
Anti-TubulinLoadingControlAntibodywaspreparedfromwholerabbitserumproducedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoaminoacids427-441ofHumanalphaTubulin.
ImmunogenType
Peptide
StorageCondition
StoreAnti-TubulinLoadingControlAntibodyat-20°Cpriortoopening.AliquotLoadingControlAntibodycontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.CentrifugeTubulinLoadingControlAntibodyifnotcompletelyclearafterstandingatroomtemperature.ThisControlAntibodyisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
Anti-TubulinAntibodyhasbeentestedforuseinELISAandwesternblot. Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Expectabandat~50kDainsizecorrespondingtoalphatubulinbywesternblottinginmostcelllysatesorextracts.
Purity/Specifity
Anti-TubulinLoadingControlAntibodyisdirectedagainsthumanalphaTubulinprotein. TheLoadingControlAntibodywasaffinitypurifiedfrommonospecificantiserumbyimmunoaffinitypurification. ABLASTanalysiswasusedtosuggestthatthisantibodywouldreactwithalphaTubulinfromawiderangeoforganisms,includingavian,mammalianaquatic,parasiticandalgasourcesbasedon100%homologyfortheimmunogensequence.Crossreactivitywilloccurwithallisoformsofalphatubulin.Suchbroadreactivitymakesthisantibodyusefulasanexcellentloadingcontrol.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
