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蚂蚁淘在线 / 品牌中心 / Rockland / Rockland/Alpha-2-Macroglobulin Antibody Peroxidase Conjugated/200-103-207-0100/100 µg
商品描述
Alpha-2-MacroglobulinAntibodyPeroxidaseConjugatedProperties Description Anti-alpha-2-MACROGLOBULIN(HumanPlasma)(GOAT)AntibodyPeroxidaseConjugated-200-103-207-0100 Host Goat TargetSpecies Human KnownCrossReactivity human Clonality Polyclonal Application ELISA:1:4,000-1:20,000 WesternBlot:1:1,000-1:5,000 Immunohistochemistry:1:500-1:2,500 OtherDilution:UserOptimized PhysicalState Lyophilized ShippingCondition Ambient Format IgG Label Peroxidase(HorserADIsh) Concentration 1mg/mLbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 ReconstitutionVolume 100µL ReconstitutionBuffer Restorewithdeionizedwater(orequivalent) Preservative 0.01%(w/v)GentamicinSulfate.DoNOTaddSodiumAzide! StABIlizer 10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree GeneName A2M RelevantLinks UniProtKB-P01023 NCBI-P01023.3 GeneID-2 |
Alpha-2-MacroglobulinAntibodyPeroxidaseConjugatedDescription
Background
Alpha2-Macroglobulinisthelargestmajornonimmunoglobulinproteininplasma.Thealpha2-macroglobulinmoleculeissynthesizedmainlyinliver,butalsolocallybymacrophages,fibroblasts,andadrenocorticalcells.Alpha2macroglobulinactsasanantiproteaseandisabletoinactivateanenormousvarietyofproteinases.Itfunctionsasaninhibitoroffibrinolysisbyinhibitingplasminandkallikrein.Itfunctionsasaninhibitorofcoagulationbyinhibitingthrombin.Alpha2-macroglobulinmayactasacarrierproteinbecauseitalsobindstonumerousgrowthfactorsandcytokines,suchasplatelet-derivedgrowthfactor,basicfibroblastgrowthfactor,TGF-ß,insulin,andIL-1ß.Isabletoinhibitallfourclassesofproteinasesbyaunique"trapping"mechanism.Thisproteinhasapeptidestretch,calledthe"baitregion"whichcontainsspecificcleavagesitesfordifferentproteinases.Whenaproteinasecleavesthebaitregion,aconformationalchangeisinducedintheproteinwhichtrapstheproteinase.Theentrappedenzymeremainsactiveagainstlowmolecularweightsubstrates(activityagainsthighmolecularweightsubstratesisgreatlyreduced).Followingcleavageinthebaitregionathioesterbondishydrolyzedandmediatesthecovalentbindingoftheproteintotheproteinase.
Synonyms
goatanti-Alpha-2-MacroglobulinAntibodyHRPConjugation,PeroxidaseConjugatedgoatanti-Alpha-2-MacroglobulinAntibody,A2Mantibody,Alpha2Mantibody,Alpha2Mantibody,CPAMD5antibody,DKFZp779B086antibody,FWP007antibody,S8637antibody
Immunogen
a2-Macroglobulin[HumanPlasma]
ImmunogenType
NativeProtein
StorageCondition
Storevialat4°Cpriortorestoration.Forextendedstoragealiquotcontentsandfreezeat-20°Corbelow.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
Anti-Alpha2-Macroglobulinhasbeenassayedagainst1.0ugofa2-Macroglobulin[HumanPlasma]inastandardcaptureELISAusingABTS(2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid])code#ABTS-100asasubstratefor30minutesatroomtemperature. Aworkingdilutionof1:12,000to1:60,000ofthereconstitutionconcentrationissuggestedforthisproduct.
Purity/Specifity
Anti-Alpha2-MacroglobulinisanIgGfractionantibodypurifiedfrommonospecificantiserumbyamulti-stepprocesswhichincludesdelipidation,saltfractionationandionexchangechromatographyfollowedbyextensivedialysisagainstthebufferstatedabove. Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-Peroxidase,anti-GoatSerumaswellaspurifiedandpartiallypurifieda2-Macroglobulin[HumanPlasma]. Crossreactivityagainsta2-Macroglobulinfromothersourcesisunknown.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
