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蚂蚁淘在线 / 品牌中心 / Rockland / Rockland/Alkaline Phosphatase Antibody Peroxidase Conjugated/200-4334-0100/100 µg
商品描述
AlkalinePhosphataseAntibodyPeroxidaseConjugatedProperties Description Anti-ALKALINEPHOSPHATASE(E.coli)(RABBIT)AntibodyPeroxidaseConjugated-200-4334-0100 Host Rabbit TargetSpecies Escherichiacoli KnownCrossReactivity E.coli Clonality Polyclonal Application PhysicalState Lyophilized ShippingCondition Ambient Format IgG Label Peroxidase(HorserADIsh) Concentration 1.0mg/mLbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 ReconstitutionVolume 100µL ReconstitutionBuffer Restorewithdeionizedwater(orequivalent) Preservative 0.01%(w/v)GentamicinSulfate.DoNOTaddSodiumAzide! StABIlizer 10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree GeneName phoA RelevantLinks UniProtKB-P00634 NCBI-P00634.1 GeneID-945041 |
AlkalinePhosphataseAntibodyPeroxidaseConjugatedDescription
Background
Alkalinephosphatase(ALP,ALKP)isahydrolaseenzymeresponsIBLeforremovingphosphategroupsfrommanytypesofmolecules,includingnucleotides,proteins,andalkaloids.Theprocessofremovingthephosphategroupiscalleddephosphorylation.Allmammalianalkalinephosphataseisoenzymesexceptplacental(PALPandSEAP)areinhibitedbyhomoarginine,and,insimilarmanner,allexcepttheintestinalandplacentalonesareblockedbylevamisole.Heatingfor~2hoursat65°CinactivatedmostisoenzymesexceptPlacentalisoforms(PALPandSEAP).
Synonyms
rabbitanti-AlkalinePhosphataseAntibodyPeroxidaseConjugation,HRPconjugatedrabbitanti-AlkalinePhosphataseAntibody,Akp2antibody,ALPantibody,Nagaoisozymeantibody,Placentalalkalinephosphataseantibody,Placentallikealkalinephosphataseantibody,PLAP1antibody,PLAPlikeantibody,Reganisozymeantibody,Kasaharaisozymeantibody
Immunogen
AlkalinePhosphatase[E.coli]
ImmunogenType
NativeProtein
StorageCondition
Storevialat4°Cpriortorestoration. Forextendedstoragealiquotcontentsandfreezeat-20°Corbelow. Avoidcyclesoffreezingandthawing. Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature. Thisproductisstableforseveralweeksat4°Casanundilutedliquid. Diluteonlypriortoimmediateuse.
ApplicationNote
Anit-alkalinephosphatasehasbeenassayedagainst1.0ugofAlkalinePhosphatase[E.coli]inastandardcaptureELISAusingABTS(2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid])code#ABTS-100asasubstratefor30minutesatroomtemperature. Aworkingdilutionof1:3,500to1:15,000ofthereconstitutionconcentrationissuggestedforthisproduct.
Purity/Specifity
AlkalinephosphataseisanIgGfractionantibodypurifiedfrommonospecificantiserumbyamulti-stepprocesswhichincludesdelipidation,saltfractionationandionexchangechromatographyfollowedbyextensivedialysisagainstthebufferstatedabove. Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-Peroxidase,anti-RabbitSerumaswellaspurifiedandpartiallypurifiedAlkalinePhosphatase[E.coli]. NocrossreactivityocursagainstmammalianformsofAlkalinePhosphatase.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
