品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
AlkalinePhosphataseAntibodyProperties Description Anti-ALKALINEPHOSPHATASE(E.coli)(RABBIT)Antibody-200-4134-0100 Host Rabbit TargetSpecies Escherichiacoli KnownCrossReactivity E.coli Clonality Polyclonal Application ELISA:1:4,000-1:20,000 WesternBlot:1:500-1:2,000 PhysicalState Lyophilized ShippingCondition Ambient Format IgG Label Unconjugated Concentration 1.0mg/mlbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 ReconstitutionVolume 100µL ReconstitutionBuffer Restorewithdeionizedwater(orequivalent) Preservative 0.01%(w/v)SodiumAzide StABIlizer None GeneName phoA RelevantLinks UniProtKB-P00634 NCBI-P00634.1 GeneID-945041 |
AlkalinePhosphataseAntibodyDescription
Synonyms
rabbitanti-AlkalinePhosphataseAntibody,Akp2antibody,ALPantibody,Nagaoisozymeantibody,Placentalalkalinephosphataseantibody,Placentallikealkalinephosphataseantibody,PLAP1antibody,PLAPlikeantibody,Reganisozymeantibody,Kasaharaisozymeantibody
Immunogen
AlkalinePhosphatase[E.coli]
ImmunogenType
NativeProtein
StorageCondition
Storevialat4°Cpriortorestoration. Forextendedstoragealiquotcontentsandfreezeat-20°Corbelow. Avoidcyclesoffreezingandthawing. Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature. Thisproductisstableforseveralweeksat4°Casanundilutedliquid. Diluteonlypriortoimmediateuse.
ApplicationNote
ALKALINEPHOSPHATASEAntibodyhasbeenassayedagainst1.0ugofAlkalinePhosphatase[E.coli]inastandardELISAusingPeroxidaseconjugatedAffinityPurifiedanti-RabbitIgG[H&L](Goat)code#611-1302and(ABTS(2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid])code#ABTS-100asasubstratefor30minutesatroomtemperature. Aworkingdilutionof1:25,000to1:125,000ofthereconstitutionconcentrationissuggestedforthisproduct. A1:10,000dilutionhasbeenreportedtobeusefulforthedetectionbyimmunoblotofanintegralmembraneproteinfromagram-positiveorganismexpressedasaLacZ/PhoAchimericderivativeinE.coli. Theresearchershoulddetermineoptimaltitersforotherapplications.
Purity/Specifity
Anti-ALKALINEPHOSPHATASEisanIgGfractionantibodypurifiedfrommonospecificantiserumbyamulti-stepprocesswhichincludesdelipidation,saltfractionationandionexchangechromatographyfollowedbyextensivedialysisagainstthebufferstatedabove. Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-RabbitSerumaswellaspurifiedandpartiallypurifiedAlkalinePhosphatase[E.coli]. CrossreactivityagainstAlkalinePhosphatasefromothertissuesandspeciesmayoccurbuthavenotbeenspecificallydetermined. SomeminimalcrossreactivityhasbeenreportedwhenlysatesofcellextractsoverproducingE.coliproteinsareprobed.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
