
品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com

商品描述
AKT2PEAntibodyProperties![]() Description Anti-AKT2(RAT)PhycoerythrinConjugatedMonoclonalAntibody-200-508-E71 Host Rat TargetSpecies Human KnownCrossReactivity Human Clonality Monoclonal16G11.E8IgG2akappa Application ELISA:UserOptimized IFMicroscopy:Useroptimized FlowCytometry:UserOptimized WesternBlot:UserOptimized Immunohistochemistry:Useroptimized PhysicalState Lyophilized ShippingCondition WetIce Format IgG2a Label R-Phycoerythrin(RPE) FPValue 1-2molesR-Phycoerythrin(RPE)permoleofIgG2a Concentration 1.0mg/mLbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.5MSodiumChloride,pH7.2 ReconstitutionVolume 50µL ReconstitutionBuffer Restorewithdeionizedwater(orequivalent) Preservative None StABIlizer 10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree GeneName AKT2 RelevantLinks UniProtKB-P31751 NCBI-NP_001617.1 GeneID-208 |
AKT2PEAntibodyDescription

Background
AKTisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasAKT1,ProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.Anti-AKT2(RAT)PEconjugatedMonoclonalAntibodyisidealforinvestigatorsinvolvedinCellSignaling,Cancer,Neuroscience,SignalTransductionresearch.

Synonyms
ratanti-Anti-AKT2PEconjugatedantibody,Phycoerythrinconjugatedratanti-Anti-AKT2antibody,AKT2antibody,AKT-2,PKBantibody,PKBbetaantibody,PKBBETAantibody,PRKBBantibody,ProteinkinaseAkt2antibody,ProteinkinaseBbetaantibody,RAC-betaserine/threonine-proteinkinase,RAC-PK-beta

Immunogen
Anti-AKT2AntibodywasproducedinratbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtointernalresiduesofhumanAKT2protein.

ImmunogenType
Peptide

StorageCondition
Storevialat4°Cpriortorestoration.Restorewithdeionizedwater(orequivalent).Thisproductisstableat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Donotfreezeafterreconstitution.Storereagentinthedark.UsesuBDuedlightingduringhandlingandincubationofcellspriortoanalysis.

ApplicationNote
Anti-AKT2PEAntibodyissuitableforFlowCytometry,ELISA,immunohistochemistry,andwesternblotting.Expectabandapproximately56kDainsizecorrespondingtoAKT2proteinbywesternblottingintheappropriatecelllysateorextract.ThismonoclonalantibodyreactswithhumanAKT.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Forimmunohistochemistrywerecommendtheuseoffreshfrozentissues.Attemptsatstainingparaffin-embeddedformalinfixedtissueswerenegative.Nopre-treatmentofsampleisrequired.

Purity/Specifity
Anti-AKT2antibodyisdirectedagainsthumanAKT2.Theantibodydetectsbothunphosphorylatedandphosphorylatedformsoftheprotein.Anti-AKT2waspurifiedfromconcentratedtissueculturesupernatebyProteinGchromatography.CrossreactivitywithAKT2fromotherspecieshasnotbeendetermined,however,thesequenceoftheimmunogenshows88%identitytomouseand90%identitywithrat,therefore,crossreactivityisexpected.

DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
