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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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商品描述
AKT2APCAntibodyProperties Description Anti-AKT2(RAT)AllophycocyaninConjugatedMonoclonalAntibody-200-526-J35 Host Rat TargetSpecies Human KnownCrossReactivity Human Clonality Monoclonal11F6.B2IgG2akappa Application ELISA:UserOptimized IFMicroscopy:Useroptimized FlowCytometry:UserOptimized WesternBlot:UserOptimized Immunohistochemistry:Useroptimized PhysicalState Lyophilized ShippingCondition WetIce Format IgG2a Label Allophycocyanine FPValue 1-2molesAllophycocyaninepermoleofIgG2a Concentration 1.0mg/mLbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.5MSodiumChloride,pH7.2 ReconstitutionVolume 50µL ReconstitutionBuffer Restorewithdeionizedwater(orequivalent) Preservative None StABIlizer 10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree GeneName AKT2 RelevantLinks UniProtKB-P31751 NCBI-NP_001617.1 GeneID-208 |
AKT2APCAntibodyDescription
Background
AKTisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasAKT1,ProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.Anti-AKT2(RAT)APCconjugatedMonoclonalAntibodyisidealforinvestigatorsinvolvedinCellSignaling,Cancer,Neuroscience,SignalTransductionresearch.
Synonyms
ratanti-Anti-AKT2AllophycocyaninConjugation,APCConjugatedratanti-Anti-AKT2,AKT2antibody,AKT-2,PKBantibody,PKBbetaantibody,PKBBETAantibody,PRKBBantibody,ProteinkinaseAkt2antibody,ProteinkinaseBbetaantibody,RAC-betaserine/threonine-proteinkinase,RAC-PK-beta
Immunogen
Anti-AKT2AntibodywasproducedinratbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtointernalresiduesofhumanAKT2protein.
ImmunogenType
Peptide
StorageCondition
Storevialat4°Cpriortorestoration.Restorewithdeionizedwater(orequivalent).Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Forextendedstorage,aliquotcontentsandfreezeat-20°Corbelow.Avoidcyclesoffreezingandthawing.Storereagentinthedark.UsesuBDuedlightingduringhandlingandincubationofcellspriortoanalysis.
ApplicationNote
Anti-AKT2APCAntibodyissuitableforFlowCytometry,ELISA,immunohistochemistry,andwesternblotting.Expectabandapproximately56kDainsizecorrespondingtoAKT2proteinbywesternblottingintheappropriatecelllysateorextract.ThismonoclonalantibodyreactswithhumanAKT.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Forimmunohistochemistrywerecommendtheuseoffreshfrozentissues.Attemptsatstainingparaffin-embeddedformalinfixedtissueswerenegative.Nopre-treatmentofsampleisrequired.
Purity/Specifity
Anti-AKT2antibodyisdirectedagainsthumanAKT2.Theantibodydetectsbothunphosphorylatedandphosphorylatedformsoftheprotein.Anti-AKT2waspurifiedfromconcentratedtissueculturesupernatebyProteinGchromatography.CrossreactivitywithAKT2fromotherspecieshasnotbeendetermined,however,thesequenceoftheimmunogenshows88%identitytomouseand90%identitywithrat,therefore,crossreactivityisexpected.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
