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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
AKT1PEAntibodyProperties Description Anti-AKT1(MOUSE)PhycoerythrinConjugatedMonoclonalAntibody-200-308-J34 Host Mouse TargetSpecies Human KnownCrossReactivity Human,Mouse,Rat Clonality Monoclonal5E5.F5.D7IgG2akappa Application ELISA:UserOptimized IFMicroscopy:Useroptimized FlowCytometry:1.0µg/mL WesternBlot:UserOptimized Immunohistochemistry:Useroptimized PhysicalState Lyophilized ShippingCondition WetIce Format IgG2a Label R-Phycoerythrin(RPE) FPValue 1-2molesR-Phycoerythrin(RPE)permoleofIgG2a Concentration 1.0mg/mLbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.5MSodiumChloride,pH7.2 ReconstitutionVolume 50µL ReconstitutionBuffer Restorewithdeionizedwater(orequivalent) Preservative None StABIlizer 10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree GeneName AKT1 RelevantLinks UniProtKB-P31749 NCBI-NP_001014431.1 GeneID-207 |
AKT1PEAntibodyDescription
Background
AKTisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasAKT1,ProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.Anti-AKT1(MOUSE)PEconjugatedMonoclonalAntibodyisidealforinvestigatorsinvolvedinCellSignaling,Cancer,Neuroscience,SignalTransductionresearch.
Synonyms
mouseanti-AKT1antibodyPEconjugation,Phycoerythrinconjugatedmouseanti-AKT-1antibody,AKT-1,PKBantibody,PKBgammaantibody,PKBGAMMAantibody,PRKBGantibody,ProteinkinaseAkt1antibody,ProteinkinaseBgammaantibody,RAC-gammaserine/threonine-proteinkinase,RAC-PK-gamma
Immunogen
Anti-AKT1AntibodywasproducedinmicebyrepeatedimmunizationswithasyntheticpeptidecorrespondingtointernalresiduesofhumanAKT1proteinfollowedbymonoclonaldevelopment.
ImmunogenType
Peptide
StorageCondition
Storevialat4°Cpriortorestoration.Restorewithdeionizedwater(orequivalent).Thisproductisstableat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Donotfreezeafterreconstitution.Storereagentinthedark.UsesuBDuedlightingduringhandlingandincubationofcellspriortoanalysis.
ApplicationNote
Anti-AKT1PEAntibodyissuitableforFlowCytometry,ELISA,immunohistochemistry,andwesternblotting.Expectabandapproximately56kDainsizecorrespondingtoAKT1proteinbywesternblottingintheappropriatecelllysateorextract.ThismonoclonalantibodyreactswithhumanAKT.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Forimmunohistochemistrywerecommendtheuseoffreshfrozentissues.Attemptsatstainingparaffin-embeddedformalinfixedtissueswerenegative.Nopre-treatmentofsampleisrequired.
Purity/Specifity
Anti-AKT1antibodyisdirectedagainsthumanAKT1.Theantibodydetectsbothunphosphorylatedandphosphorylatedformsoftheprotein.Anti-AKT1antibodywaspurifiedfromascitesbyProteinAchromatography.CrossreactivitywithAKT1fromotherspecieshasnotbeendetermined,however,thesequenceoftheimmunogenshows85%identitytomouseand92%identitywithrat,therefore,crossreactivityisexpected.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
