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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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商品描述
AKT1AntibodyProperties Description Anti-AKT1(MOUSE)MonoclonalAntibody-200-301-I51 Host Mouse TargetSpecies Human KnownCrossReactivity human Clonality Monoclonal14E5.A2.B2.H9IgG2akappa Application ELISA:1:2,000-1:10,000 FlowCytometry:UserOptimized WesternBlot:1:1000 Immunohistochemistry:20µg/mL OtherDilution:UserOptimized PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format IgG2a Label Unconjugated Concentration 1.0mg/mlbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 Preservative 0.01%(w/v)SodiumAzide StABIlizer None GeneName AKT1 |
AKT1AntibodyDescription
Background
AKT1AntibodydetectsAKT1whichisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.Anti-AKT1AntibodyisidealforinvestigatorsinvolvedinCellSignaling,NeuroscienceandSignalTransductionresearch.
Synonyms
mouseanti-AKT1antibody,AKT-1,PKBantibody,PKBgammaantibody,PKBGAMMAantibody,PRKBGantibody,ProteinkinaseAkt1antibody,ProteinkinaseBgammaantibody,RAC-gammaserine/threonine-proteinkinase,RAC-PK-gamma
Immunogen
Anti-AKT1AntibodywasproducedinmicebyrepeatedimmunizationswithasyntheticpeptidecorrespondingtointernalresiduesofhumanAKT1proteinfollowedbymonoclonaldevelopment.
ImmunogenType
Peptide
StorageCondition
Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
Anti-AKT1AntibodyissuitableforELISA,immunohistochemistry,flowcytometry,andwesternblotting.Expectabandapproximately56kDainsizecorrespondingtoAKT1proteinbywesternblottingintheappropriatecelllysateorextract.ThismonoclonalantibodyreactswithhumanAKT.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Forimmunohistochemistrywerecommendtheuseoffreshfrozentissues.Attemptsatstainingparaffin-embeddedformalinfixedtissueswerenegative.Nopre-treatmentofsampleisrequired.
Purity/Specifity
Anti-AKT1antibodyisdirectedagainsthumanAKT1.Theantibodydetectsbothunphosphorylatedandphosphorylatedformsoftheprotein.Anti-AKT1antibodywaspurifiedfromascitesbyProteinAchromatography.CrossreactivitywithAKT1fromotherspecieshasnotbeendetermined,however,thesequenceoftheimmunogenshows85%identitytomouseand92%identitywithrat,therefore,crossreactivityisexpected.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
