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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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商品描述
AKTpS473AntibodyProperties Description Anti-AKTpS473(RABBIT)Antibody-600-401-268 Host Rabbit TargetSpecies Human KnownCrossReactivity Human,Mouse,Rat Clonality Polyclonal Application ELISA:1:15,000-1:60,000 FlowCytometry:UserOptimized WesternBlot:1:200-1:1000 Immunohistochemistry:1:100-1:500 OtherDilution:UserOptimized PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format IgG Label Unconjugated Concentration 1.0mg/mLbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 Preservative 0.01%(w/v)SodiumAzide StABIlizer None GeneName AKT1 RelevantLinks NCBI-62241011 UniProtKB-P31749 GeneID-207 |
AKTpS473AntibodyDescription
Background
AKTisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationat Ser473andThr308. AKTisacytoplasmicproteinalsoknownasAKT1,ProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways. AKTExhibitstightcontrolovercellproliferationandcellviability. OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer. AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephos-phorylated,AKTdissociatesfromthemembraneandphos-phorylatestargetsinthecytoplasmandthecellnucleus. AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.
Synonyms
rabbitanti-AKTpS473Antibody,AKT1phosphoS473,RAC-PK-alpha,ProteinkinaseB,PKB,C-AKT,RAC-alphaserine/threonine-proteinkinase,Proto-oncogenec-Akt,AKT1,AKT1,AKT-1
Immunogen
ThiswholerabbitserumwaspreparedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoaC-terminusregionnearaa460-480ofhuman,mouse,ratandchickenAKTproteinsconjugatedtoKLH.
ImmunogenType
Peptide
PostTranslationalModification
Phosphorylation
StorageCondition
Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
ThisantibodyisphosphospecificforpS473andissuitableforwesternblotting,immunohistochemistry(formalin-fixedparaffin-embeddedsections),immunofluorescenceandELISA.Byimmunoblotasinglebandoftheexpectedapparentmolecularweightisobserved. Forimmunohistochemistrynopre-treatmentofsampleisrequired.
Purity/Specifity
Thisproductwaspreparedfrommonospecificantiserumbyimmunoaffinitychromatographyusingphosphopeptidecoupledtoagarosebeadsfollowedbysolidphaseadsorption(s)againstnon-phosphopeptideandnon-specificpeptidetoremoveanyunwantedreactivities.Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-RabbitSerum. ThisantibodyisspecificforphosphorylatedhumanAKTpS473. Minimalreactivityoccursagainstnon-phosphorylatedAKT. ReactivityagainstAKTfromotherspeciesmayoccurbuthasnotyetbeentested.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
