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蚂蚁淘在线 / 品牌中心 / Rockland / Rockland/Akt phospho T308 Dylight 649 Conjugated Antibody/200-343-269/100 µg
商品描述
AktphosphoT308Dylight649ConjugatedAntibodyProperties Description Anti-AKTpT308(MOUSE)MonoclonalAntibodyDyLight™649Conjugated-200-343-269 Host Mouse TargetSpecies Human KnownCrossReactivity human,mouse,rat,monkey Clonality Monoclonal18F3.H11IgG1kappa Application ELISA:1:4,000-1:20,000 FLISA:>1:20,000 IFMicroscopy:>1:5,000 FlowCytometry:UserOptimized WesternBlot:>1:10,000 OtherDilution:UserOptimized PhysicalState Lyophilized ShippingCondition Ambient Format IgG1 Label DyLight™649 FPValue 1.3molesDyLight™649permoleofIgG1 Concentration 1.0mg/mLbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 ReconstitutionVolume 100µL ReconstitutionBuffer Restorewithdeionizedwater(orequivalent) Preservative 0.01%(w/v)SodiumAzide StABIlizer 10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree GeneName AKT1 RelevantLinks UniProtKB-P31749 NCBI-62241011 GeneID-207 |
AktphosphoT308Dylight649ConjugatedAntibodyDescription
Background
AKTisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasAKT1,ProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.
Synonyms
mouseanti-AKTpT308DyLight™649conjugatedAntibody,DyLight™649conjugatedmouseanti-AKTpT308Antibody,RAC-PK-alpha,ProteinkinaseB,PKB,C-AKT,RAC-alphaserine/threonine-proteinkinase,Proto-oncogenec-Akt,AKT1,AKT1,AKT-1
Immunogen
ThismonoclonalantibodywasproducedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoresiduessurroundingT308ofhumanAKT1protein.
ImmunogenType
Peptide
PostTranslationalModification
Phosphorylation
StorageCondition
Storevialat4°Cpriortorestoration. Forextendedstoragealiquotcontentsandfreezeat-20°Corbelow. Avoidcyclesoffreezingandthawing. Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature. Thisproductisstableforseveralweeksat4°Casanundilutedliquid. Diluteonlypriortoimmediateuse.
ApplicationNote
Thismonoclonalantibodyissuitableforimmunofluorescencemicroscopy,FLISAandwesternblotting.Expectabandapproximately56kDainsizecorrespondingtophosphorylatedAKTproteinbywesternblottingintheappropriatecelllysateorextract.Thisphospho-specificmonoclonalantibodyreactswithhumanandmouseAKTpT308andshowsminimalreactivitybyELISAagainstthenon-phosphorylatedformoftheimmunizingpeptide.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.
Purity/Specifity
ThisproductwaspurifiedfromconcentratedtissueculturesupernatebyProteinAchromatography.ThisantibodyisspecificforhumanandmouseAKTproteinphosphorylatedatT308.ABLASTanalysiswasusedtosuggestcross-reactivitywithAKTpT308frommostvertebratespeciessourcesbasedon100%homologywiththeimmunizingsequence.Cross-reactivitywithAKTfromothersourceshasnotbeendetermined.Cross-reactivitywithAKT2andAKT3willlikelyoccur.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
