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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
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https://www.ebiomall.com
商品描述
AgoAntibodyProperties Description Anti-Ago(Argonautes)(MOUSE)MonoclonalAntibody-200-301-V04 Host Mouse TargetSpecies Human KnownCrossReactivity Human,Mouse,Other(widerange) Clonality Monoclonal2A8IgG1 Application IFMicroscopy:1:500 WesternBlot:1:300-1:500 Immunohistochemistry:1:500 ImmunoPrecipitation:8µg OtherDilution:HITS-CLIP:UserOptimizedDilution PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format IgG1 Label Unconjugated Concentration 2.0mg/mlbyUVabsorbanceat280nm Buffer 0.01MSodiumPhosphate,0.25MSodiumChloride,pH7.2 Preservative 0.05%(w/v)SodiumAzide StABIlizer None GeneName AGO2 RelevantLinks UniProtKB-Q9UKV8 GeneID-27161 NCBI-NP_001158095 |
AgoAntibodyDescription
Background
MembersoftheArgonaute(Ago)proteinfamilyareinternaltoRISCfunction.ArgonauteproteinsbindtomaturemicroRNA(miRNAs)andshortinterferingRNAs(siRNAs)andformthecorecomponentsofeffectorcomplexesthatmediatemiRNAandsiRNAfunction.ArgonautesareneededformiRNA-inducedsilencingandcontaintwoconservedRNAbindingdomains:aPAZdomainthatcanbindthesinglestranded3’endofthematuremiRNAandaPIWIdomainthatstructurallyresemblesribonuclease-Handfunctionstointeractwiththe5’endoftheguidestrand.Someargonautes,forexamplehumanAgo2,cleavetargettranscriptsdirectly;argonautesmayalsorecruitadditionalproteinstoachievetranslationalrepression.ThehumangenomeencodeseightArgonauteproteinsdividedbysequencesimilaritiesintotwofamilies:AGO(withfourmemberspresentinallmammaliancellsandcalledE1F2C/hAgoinhumans),andPIWI(foundinthegermlineandhematopoieticstemcells).Anti-AgoAntibodyisidealforresearchinAlternativeSplicing,GeneExpressionandGeneSilencing.
Synonyms
Proteinargonaute-2,Argonaute2,hAgo2,ArgonauteRISCcatalyticcomponent2,Eukaryotictranslationinitiationfactor2C2,eIF-2C2,eIF2C2,PAZPiwidomainprotein,PPD,Proteinslicer
Immunogen
Anti-AgoAntibodywasproducedinmicebyrepeatedimmunizationwithhumanAgo2protein(fusionproteincontainingaminoacids47-859ofhumanAgo2).
ImmunogenType
Peptide
StorageCondition
Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
Anti-AgoAntibodyissuitableforHITS-CLIP(High-throughputsequencingofRNAisolatedbycrosslinkingimmunoprecipitation),Immunofluorescence,Immunohistochemistry,ImmunoprecipitationandWesternBlots.Specificconditionsforreactivityshouldbeoptimizedbytheenduser. Expectabandapproximately97.2kDaintheappropriatecelllysateorextract.
Purity/Specifity
Anti-AgoAntibodywaspurifiedbyProteinAchromatography.This2A8monoclonalantibodyrecognizesall4humanandmouseAgoproteinswhilehavinghigheraffinityforAgo2.Cross-reactivitywithAgo2fromothersourceshasnotbeendetermined.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
