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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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商品描述
ACSS2AntibodyProperties Description Anti-ACSS2(RABBIT)Antibody-600-401-GW7S Host Rabbit TargetSpecies Human KnownCrossReactivity human Clonality Polyclonal Application ELISA:1:10,000-1:50,000 WesternBlot:1.0ug/ml PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format IgG Label Unconjugated Concentration 1.06mg/mlbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 Preservative 0.01%(w/v)SodiumAzide StABIlizer None GeneName ACSS2 Paired No CellViability No RelevantLinks GeneID-55902 NCBI-NP_001070020.2 UniProtKB-Q9NR19 |
ACSS2AntibodyDescription
Background
Thisantibodyisdesigned,produced,andvalidatedaspartofacollaborationbetweenRocklandandtheNationalCancerInstitute(NCI).ACSS2(Acyl-CoAsynthetaseshort-chainfamilymember2)isaproteincodinggeneencodingacytosolicenzymethatcatalyzestheactivationofacetateforuseinlipidsynthesisandenergygeneration.Thisproteinactsasamonomerandproducesacetyl-CoAfromacetateinareactionthatrequiresATP.Expressionofthisgeneisregulatedbysterolregulatoryelement-bindingproteins,transcriptionfactorsthatactivategenesrequiredforthesynthesisofcholesterolandunsaturatedfattyacids.Thisproteinisupregulatedinmanycancersandisimportantforpro-survivalunderhypoxicconditions.ACSS2maybeassociatedwithdisorderssuchasCystoisosporiasis.Anti-ACSS2Antibodyisusefulforresearchersinterestedinparasites,intestines,GlucoseEnergyMetabolism,andAKTresearch.
Synonyms
rabbitanti-ACSS2antibody,ACSS-2,ACSS2,Acetyl-coenzymeAsynthetase(cytoplasmic),Acetate-CoAligase,Acetyl-CoAsynthetase,ACS,AceCS,Acyl-CoAsynthetaseshort-chainfamilymember2,Acyl-activatingenzyme,ACAS2
Immunogen
ACSS2affinitypurifiedantibodywaspreparedfromwholerabbitserumproducedbyrepeatedimmunizationswithsyntheticpeptideneartheN-terminusofhumanACSS2.
ImmunogenType
Peptide
StorageCondition
Storevialat-20°Corbelowpriortoopening.Thisvialcontainsarelativelylowvolumeofreagent(25µL).Tominimizelossofvolumedilute1:10byadding225µLofthebufferstatedabovedirectlytothevial.Recap,mixthoroughlyandbrieflycentrifugetocollectthevolumeatthebottomofthevial.Usethisintermediatedilutionwhencalculatingfinaldilutionsasrecommendedbelow.Storethevialat-20°Corbelowafterdilution.Avoidcyclesoffreezingandthawing.
ApplicationNote
Anti-ACSS2AntibodyhasbeentestedinWesternBlotandELISA.Expectabandatapproximately76kDainwesternblotusingappropriatelysates.PositivecontrolwholecelllysatesusedHep-G2at1µg/mLforWB.Althoughnottested,thisantibodyislikelyfunctionalinimmunohistochemistry,immunofluorescence,andimmunoprecipitation.
Purity/Specifity
ThisaffinitypurifiedantibodyisdirectedagainsthumanACSS2protein.Anti-ACSS2Antibodytowasaffinitypurifiedfrommonospecificantiserumbyimmunoaffinitychromatography.Blastanalysisofthesequenceoftheimmunogenshows100%identitywithhumanand80%identitytomouse.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
