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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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商品描述
Aak1AntibodyPropertiesDescription Anti-Aak1(RABBIT)Antibody-600-401-X53 Host Rabbit TargetSpecies Human KnownCrossReactivity Human,mouse,rat Clonality Polyclonal Application ELISA:1:10,000-1:20,000 WesternBlot:1-2µg/mL PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format IgG Label Unconjugated Concentration 1mg/mLbyUVabsorbanceat280nm Buffer 0.01MSodiumPhosphate,0.25MSodiumChloride,pH7.2 Preservative 0.02%(w/v)SodiumAzide StABIlizer None GeneName AAK1 RelevantLinks UniProtKB-Q2M2I8 GeneID-22848 NCBI-NP_055726 |
Aak1AntibodyDescription
Background
AP2-associatedproteinkinase1(Aak1)isamemberoftheArk1/Prk1subfamilyofSer/Thrproteinkinasesthatarethoughttoregulateendocytosisbyphosphorylatingtheaccessoryendocyticcomponents.Aak1interactswithandphosphorylatesthemu2subunitoftheAP-2complex,whichpromotesbindingoftheAP-2totyrosinebased(Yxxphi)internalizationmotif-containingreceptorsandsubsequentreceptorendocytosis.AtleasttwoisoformsofAak1areknowntoexist;thelongerisoformcontainsanextendedcarboxy-terminusthatcontainsanadditionalclathrin-bindingdomain.OverexpressionofthislongisoformorAak1depletionbyRNAinterferenceimpairstransferrinrecyclingfromtheearly/sortingendosome,suggestingthatAak1functionsatmultiplestepsoftheendosomalpathwaybyregulatingtransferrininternalizationanditsrecyclingbacktotheplasmamembrane.
Synonyms
Aak1Antibody,KIAA1048,AP2-associatedproteinkinase1,Adaptor-associatedkinase1
Immunogen
Anti-Aak1antibodywaspreparedfromwholerabbitserumproducedbyrepeatedimmunizationswitha18aminoacidsyntheticpeptideneartheC-terminusofthehumanAak1.
ImmunogenType
Peptide
StorageCondition
Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
Anti-Aak1AntibodyhasbeentestedforuseinWesternBlotting.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Expectabandat~104kDainsizecorrespondingtoAak1proteinbyWesternBlottingintheappropriatecelllysateorextract.
Purity/Specifity
Anti-Aak1Antibodywasaffinitypurifiedfrommonospecificantiserumbyimmunoaffinitychromatography.CrossreactivitywithAak1fromothersourceshasnotbeendetermined.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.