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商品描述
8-HydroxyGuanineAntibodyProperties Description Anti-8-HydroxyGuanine(MOUSE)MonoclonalAntibody-200-301-A99 Host Mouse KnownCrossReactivity 8hydroxy2’deoxyguanosine,8-hydroxyguanineand8-hydroxyguanosine Clonality Monoclonal15A3IgG2a Application PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format IgG2a Label Unconjugated Concentration 1.0mg/mLbyUVabsorbanceat280nm Buffer 0.01MSodiumPhosphate,0.15MSodiumChloride,pH7.2 Preservative 0.1%(w/v)SodiumAzide StABIlizer 50%(v/v)Glycerol |
8-HydroxyGuanineAntibodyDescription
Background
DNAorRNAdamageisduetoenvironmentalfactorsandnormalmetabolicprocessesinsidethecell,thatthenhindertheabilityofthecelltocarryoutitsfunctions.TherearefourmaintypesofDNAdamageduetoendogenouscellularprocesses:oxidation,alkylation,hydrolysisandmismatchofthebases.Duringtheoxidationofbases,highlyreactivechemicalentitiescollectivelyknownasRONSmaydevelop.RONSstandsforreactiveoxygenandnitrogenspeciesandincludesnitricoxide,superoxide,hydroxylrADIcal,hydrogenperoxideandperoxynitrite.NumerousstudieshaveshownthatRONScauseavarietyofotherissuesinadditiontoDNAdamage(1).8-hydroxyguanine,8-hydroxy-2’-deoxyguanosineand8-hydroxyguanosineareallRNAandDNAMarkersofoxidativedamage.8-hydroxy-2’-guanosineisproducedbyreactiveoxygenandnitrogenspeciesincludinghydroxylradicalandperoxynitrite.SpecificallyitshighBIOLOGicalrelevanceisduetoitsabilitytoinduceGtoTtransversions,whichisoneofthemostfrequentsomaticmutations(2).8-hydroxy-guaninehasbeenthemostfrequentlystudiedtypeofDNAbasedamage,withstudiesindiabetes,andcancer.Basemodificationsofthistypearisefromradical-inducedhydroxylationandcleavagereactionsofthepurinering(3,4).Finally,8-hydroxy-guanosine,like8-hydroxy-2’-guanosine,inducesamutagenictransversionofGtoTinDNA.Itsrolehasbeentestedspecificallyinthedevelopmentofdiabetes,hypertensionandstrokes(5,6,and7).
Synonyms
8hydroxy2’deoxyguanosineantibody,8hydroxyguanineantibody,8hydroxyguanosineantibody,8OHGantibody,8-OHGantibody,8OGantibody,8OHdGantibody,8OHGantibody
Immunogen
ThisProteinGpurifiedmonoclonalantibodywaspreparedusingconventionalhybridomatechnologyafterrepeatedimmunizationswith8-hydroxy-guanosine-BSAandcaseinconjugates.
ImmunogenType
NativeProtein
StorageCondition
StoreAnti-8-HydroxyGuanineantibodyat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
ThisProteinGpurifiedantibodyhasbeentestedforuseinimmunohistochemistryandELISA. Specificconditionsforreactivityshouldbeoptimizedbytheenduser.
Purity/Specifity
ThisProteinGpurifiedAnti-8-HydroxyGuaninemonoclonalantibodyrecognizesmarkersofoxidativedamagetoDNA(8-hydroxy-2"-deoxyguanosine,8-hydroxyguanineand8-hydroxyguanosine).
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
