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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
5hmcAntibodyPropertiesDescription Anti-5-hydroxymethylcytosine(MOUSE)MonoclonalAntibody-200-301-V06 Host Mouse TargetSpecies Human KnownCrossReactivity Human,Mouse,Other(widerange) Clonality MonoclonalIgG1kappa Application PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format IgG1 Label Unconjugated Concentration 1.0mg/mlbyUVabsorbanceat280nm Buffer 0.01MSodiumPhosphate,0.25MSodiumChloride,pH7.2 Preservative 0.05%(w/v)SodiumAzide StABIlizer None |
5hmcAntibodyDescription
Background
5-hydroxymethylcytosine(5-hmC)hasbeenrecentlydiscoveredinmammalianDNA.Thisresultsfromtheenzymaticconversionof5-methylcytosineinto5-hydroxymethylcytosinebytheTETfamilyofoxygenases.Sofar,the5-hmCbaseshavebeenidentifiedinPurkinjeneurons,ingranulecellsandembryonicstemcellswheretheyarepresentathighlevels(upto0,6%oftotalnucleotidesinPurkinjecells).Preliminaryresultsindicatethat5-hmCmayhaveimportantrolesdistinctfrom5-mC.Althoughitspreciserolehasstilltobeshown,earlyevidencesuggestsafewputativemechanismsthatcouldhavebigimplicationsinepigenetics:5-hydroxymethylcytosinemaywellrepresentanewpathwaytodemethylateDNAinvolvingarepairmechanismconverting5-hmCtocytosineand,assuchopenupentirelynewperspectivesinepigeneticstudies.Duetothestructuralsimilaritybetween5-mCand5-hmC,thesebasesareexperimentallyalmostindistinguishable.Recentarticlesdemonstratedthatthemostcommonapproaches(e.g.enzymaticapproaches,bisulfitesequencing)donotaccountfor5-hmC.Thedevelopmentoftheaffinity-basedtechnologiesappearstobethemostpowerfulwaytodifferentiallyandspecificallyenrich5-mCand5-hmCsequences.Anti-5hmCAntibodyisidealforresearchinEpigeneticsandGeneExpression.
Synonyms
5-hydroxymethylcytosine
Immunogen
Anti-5hmCAntibodywasproducedinmicebyrepeatedimmunizationwith5-hydroxymethlcytosine.
ImmunogenType
Other
StorageCondition
Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
Anti-5hmCAntibodyissuitableforDotBlotting,ELISA,HydroxymethylatedDNAImmunoprecipitationSequencingandImmunofluorescence.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.
Purity/Specifity
Anti-5hmCAntibodywaspurifiedbyProteinAchromatography.Cross-reactivitywith5hmCfromothersourceshasnotbeendetermined.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.