品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
4E-BP1AntibodyProperties Description Anti-4E-BP1(RABBIT)Antibody-600-401-X52 Host Rabbit TargetSpecies Human KnownCrossReactivity Human,mouse Clonality Polyclonal Application PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format IgG Label Unconjugated Concentration 1mg/mLbyUVabsorbanceat280nm Buffer 0.01MSodiumPhosphate,0.25MSodiumChloride,pH7.2 Preservative 0.02%(w/v)SodiumAzide StABIlizer None GeneName EIF4EBP1 RelevantLinks UniProtKB-Q13541 GeneID-1978 NCBI-NP_004086.1 |
4E-BP1AntibodyDescription
Background
ThetranslationofmRNAineukaryoticcellsisregulatedbythepresenceofaminoacidsthroughmultiplemechanisms.Onesuchmechanisminvolvestheevolutionarilyconservedserine/threoninekinaseTOR(Targetofrapamycin,alsoknownasmTOR),whichregulatescellgrowthandcellcyclethroughitsabilitytointegratesignalsfromnutrientlevelsandgrowthfactors.OnedownstreamtargetofTORistheeukaryoticinitiationfactor4Ebindingprotein1(4E-BP1)whosephosphorylationpreventsitsassociationwitheIF4E,preferentiallystimulatingtranslationofmRNAscontaininglong,highlystructured5"-UTRs.RapamycininhibitsTORresultinginreducedcellgrowthandreducedratesofcellcycleandcellproliferationl,atleastinpartbyinhibitingtheactivityofTORtowards4E-BP1.
Synonyms
4E-BP1Antibody,BP-1,4EBP1,4E-BP1,PHAS-I,Eukaryotictranslationinitiationfactor4E-bindingprotein1,Phosphorylatedheat-andacid-stableproteinregulatedbyinsulin1
Immunogen
Anti-4E-BP1antibodywaspreparedfromwholerabbitserumproducedbyrepeatedimmunizationswitha14aminoacidsyntheticpeptidefromneartheC-terminusofhuman4E-BP1.
ImmunogenType
Peptide
StorageCondition
Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
4E-BP1affinitypurifiedantibodyhasbeentestedforuseinELISA,immunocytochemistryandwesternblotting.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Expectabandat~12.6kDainsizecorrespondingto4E-BP1bywesternblottingintheappropriatecelllysateorextract.
Purity/Specifity
Anti-4E-BP1Antibodywasaffinitypurifiedfrommonospecificantiserumbyimmunoaffinitychromatography.Crossreactivitywith4E-BP1fromothersourceshasnotbeendetermined.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
