品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
NFkBp65(RelA)PhosphoS276AntibodyProperties Description Anti-NFKBp65(RelA)pS276(RABBIT)Antibody-100-401-264 Host Rabbit TargetSpecies Human KnownCrossReactivity human Clonality Polyclonal Application ELISA:1:10,000-1:30,000 WesternBlot:1:1,000-1:5,000 Immunohistochemistry:1:200-1:800 OtherDilution:UserOptimized PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format Antiserum Label Unconjugated Concentration 80mg/mLbyRefractometry Buffer None Preservative 0.01%(w/v)SodiumAzide StABIlizer None GeneName RELA RelevantLinks UniProtKB-Q04206 NCBI-223468676 GeneID-5970 |
NFkBp65(RelA)PhosphoS276AntibodyDescription
Background
NF-kBwasoriginallyidentifiedasafactorthatbindstotheimmunoglobulinkappalightchainenhancerinBcells.Itwassubsequentlyfoundinnon-BcellsinaninactivecytoplasmicformconsistingofNF-kBboundtoIkB.NF-kBwasoriginallyidentifiedasaheterodimericDNAbindingproteincomplexconsistingofp65(RelA)andp50(NFKB1)subunits.Otheridentifiedsubunitsincludep52(NFKB2),cRel,andRelB.Thep65,cRel,andRelBsubunitsareresponsIBLefortransactivation.Thep50andp52subunitspossessDNAbindingactivitybutlimitedabilitytotransactivate.p52hasbeenreportedtoformtranscriptionallyactiveheterodimerswiththeNF-kBsubunitp65,similartop50/p65heterodimers.Lowlevelsofp52andp50homodimerscanalsoexistincells.Theheterodimersofp52/p65andp50/p65areregulatedbyphysicalinactivationinthecytoplasmbyIkB-alpha.IkB-alphabindstothep65subunitpreventingnuclearlocalizationandDNAbinding.ActivatorsmediatearapidphosphorylationofIkBbyIkBkinase(IKK)whichresultsinsubsequentubiquitinationandproteolyticdegradation.NF-kBisthentransportedtothenucleus,whereitactivatestranscriptionoftargetgenesthroughbindingtoNF-kBtargetsequenceswithinthepromoter.TheHTLV-IproteinTaxcaninduceconstitutiveNF-kBactivationthroughphosphorylationofbothIkB-alphaandIkB-beta.ThetransformingproteinTaxinhibitsp53transcriptionalactivitythroughtheNFkBsignalingpathway,specificallyviathep65(RelA)subunit.Theinhibitionofp53activityisdependentuponphosphorylationofp65(RelA)atS536bytheupstreamkinaseIKKß.Anti-NFkBantibodyisidealforCellBIOLOGy,NuclearSignaling,NeuroscienceandSignalTransductionResearch.
Synonyms
rabbitanti-NFkBp65pS276Antibody,rabbitanti-RelApS276Antibody,NFKB,nfkb,NF-kB,NF-kappaB,NFkappaB,Anti-NF-kBantibody
Immunogen
NFkBp65(RelA)peptidecorrespondingtoaninternalregionnearphosphoSerine276ofthehumanproteinconjugatedtoKeyholeLimpetHemocyanin(KLH).
ImmunogenType
Peptide
PostTranslationalModification
Phosphorylation
StorageCondition
Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
PhosphoNFkBantibodyreactshumanpS276p65andshowsminimalreactivitybywesternblotwithnon-phosphorylatedp65andminimalreactivitybyELISAagainstthenon-phosphorylatedformoftheimmunizingpeptide. A1:500dilutionhasbeenusedforstainingp65inhumankidneytissuebyIHC.Tissuewasformalinfixedandparaffinembedded.Althoughnottested,thisantibodyislikelyfunctionalinimmunohistochemistryandimmunoprecipitation.Allconditionsmustbeuseroptimized.
Purity/Specifity
Anti-NFkBp65(RelA)PhosphoS276Antibodywaspreparedfrommonospecificantiserumbydelipidationanddefibrination.ThisphosphospecificpolyclonalantibodyisspecificforphosphorylatedpS276humanp65. Reactivitywithnon-phosphorylatedp65isminimal.CrossreactivitywithpS276phosphorylatedp65frommouse,ratorotherspecieshasnotbeendetermined.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
