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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
ArfGAP3AntibodyProperties Description Anti-ArfGAP3(RABBIT)Antibody-100-401-H46 Host Rabbit TargetSpecies Human KnownCrossReactivity Human Clonality Polyclonal Application PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format Antiserum Label Unconjugated Concentration 70byRefractometry Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 Preservative 0.01%(w/v)SodiumAzide GeneName ARFGAP3 RelevantLinks UniProtKB-Q9NP61 NCBI-NP_001135765.1 GeneID-26286 |
ArfGAP3AntibodyDescription
Background
Thisantibodyisdesigned,produced,andvalidatedaspartofacollaborationbetweenRocklandandtheNationalCancerInstitute(NCI)andissuitableforCancer,ImmunologyandNuclearSignalingresearch.TheArfGAPsareafamilyofproteinsencodedby31genesinhumans.ThefunctionoftheArfGAPsistoregulatethesmallGproteinArf,aregulatorofmembranetrafficandactinCytoskeleton.TheArfGAPshavebeenfoundtobekeyregulatorsofcellularbehaviorsthatinvolvecoordinatedactinandmembraneremodeling,includingproteinsecretionandmigration.SeveralArfGAPsareassociatedwithcancercellinvasionandmetastasis,andtheArfpathwayhasbeenfoundtobeaffectedinanumberofgeneticdiseases.AlthoughtheimportanceoftheArfGAPsandtheArfpathwayforcellularphysiologyiswellrecognized,themolecularbasisforthefunctionoftheseproteinshasnotbeenestablished.Reagentsforthestudies,especiallyantibodiesspecificforparticularmembersoftheArfGAPfamilythatcanbeusedforimmunoblottingandimmunofluorescence,arenotavailable.ArfGAP3isoneofthreeArfGAPsthoughttobespecificallyassociatedwiththeGolgiapparatus;however,localizationhasonlybeendoneforectopicallyexpressedrecombinantproteinandcellularfunctionisstillnotestablished.Indeed,thereisagrowingcontroversyaboutthefunctionofArfGAP3togetherwithArfGAP1andArfGAP2.Antibodiessuitableforimmunoprecipitation,immunoblottingandimmunofluorescencewouldbeofgreatvalueinaddressingthecontroversyandfordiscoveringthepotentialroleofArfGAP3insecretionofproteins,suchasgrowthfactors,andtraffickingofotherproteinstothecellsurface,suchasgrowthfactorreceptorsandcelladhesionmolecules.
Synonyms
rabbitanti-ArfGAP3Antibody,ARFGAP1,ADP-ribosylationfactorGTPase-activatingprotein3,ARFGAP3,Arf-GAP,ArfGAP
Immunogen
ThiswholerabbitserumwaspreparedbyrepeatedimmunizationswithatruncatedrecombinantsequenceofArfGAP3fusedtoGST.
ImmunogenType
RecombinantProtein
StorageCondition
Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
ArfGAP3hasbeentestedforuseinELISA,Immunofluorescence,andwesternblotting.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Expectabandapproximately57kDainsizebywesternblottingintheappropriatecelllysateorextract.
Purity/Specifity
Anti-ArfGAP3atibodywaspreparedfrommonospecificantiserumbydelipidationanddefibrination.FurtherpurificationwasusedtoremovetheGSTtag.TheantibodydetectsArfGAP3incelllysates.ABLASTanalysiswasusedtosuggestcrossreactivitywithhuman,monkey,andorangutanforArfGAP3.Cross-reactivitywithAfGAP3fromothersourceshavenotbeendetermined.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitABIlityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
