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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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商品描述
Aquaporin2phosphoS261AntibodyProperties Description Anti-Aquaporin2pS261(Rabbit)Antibody-612-401-D08 Host Rabbit TargetSpecies Rat KnownCrossReactivity rat Clonality Polyclonal Application ELISA:1:10,000 WesternBlot:1:1000 PhysicalState Liquid ShippingCondition DryIce Format IgG Label Unconjugated Buffer 0.01MHEPES,0.15MSodiumChloride,pH7.5 StABIlizer 0.1mg/mlBovineSerumAlbumin(BSA)-IgGandProteasefree,50%(v/v)Glycerol GeneName AQP2 RelevantLinks UniProtKB-P34080 GeneID-25386 UniProtKB-P34080.1 |
Aquaporin2phosphoS261AntibodyDescription
Background
Aquaporin2pS261antibodyrecognizesaquaporin2(AQP2)whichisahormonallyregulatedwaterchannellocatedintherenalcollectingduct.MutationsintheAQP2genecausehereditarynephrogenicdiabetesinsipidusinhumans.AvasopressininducedcAMPincreaseresultsinthephosphorylationofAQP2atserine-256anditstranslocationfromtheintracellularvesiclestotheapicalmembraneofprincipalcells.Recently,serine-261hasbeenidentifiedasanovelphosphorylationsiteonAQP2andlevelsofphosphorylatedS261havebeenshowntodecreasewithvasopressintreatmentsuggestingitsinvolvementinvasopressin-dependentAQP2trafficking.Anti-Aquaporin2pS261antibodyisidealforinvestigatorsinvolvedinCellSignaling,Neuroscience,SignalTransductionresearch.
Synonyms
ADHwaterchannel,Aquaporin-CD,Collectingductwaterchannelprotein,WCH-CD,Waterchannelproteinforrenalcollectingduct
Immunogen
Anti-Aquaporin2pS261Antibodywasproducedbyrepeatedimmunizationswithsyntheticphospho-peptidecorrespondingtoaminoacidresiduessurroundingSer261.
ImmunogenType
Peptide
PostTranslationalModification
Phosphorylation
StorageCondition
Storevialat-20°Cpriortoopening. Thisproductisstableat4°Casanundilutedliquid. Forextendedstorage,aliquotcontentsandfreezeat-20°Corbelow. Avoidcyclesoffreezingandthawing.Diluteonlypriortoimmediateuse.
ApplicationNote
Anti-Aquaporin2pS261antibodyissuitableforuseinELISA,WesternBlotting.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Expectabandofapproximately29kDaAQP2proteinphosphorylatedatSer261intheappropriatecelllysateorextract.Thisantibodyalsorecognizestheglycosylatedforofaquaporin2atapproximately37kDainsize.
Purity/Specifity
Anti-Aquaporin2pS261AntibodyisdirectedagainstratAquaporin2proteinphosphorylatedatS261.Theantibodywaspreparedfrommonospecificantiserumbyimmunoaffinitychromatographyusingphosphopeptidecoupledtoagarosebeadsfollowedbysolidphaseadsorption(s)againstnon-phosphopeptideandnon-specificpeptidetoremoveanyunwantedreactivities.Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-RabbitSerum.Minimalreactivityoccursagainstnon-phosphorylatedAquaporin2.ReactivityagainstAquaporin2occursfromratsources.However,reactivityisalsoexpectedagainstthefollowingspeciesbasedon100%sequencehomology:canineandbovine,chicken,human,mouseandnon-humanprimate.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
