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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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商品描述
APG8AntibodyProperties Description Anti-APG8(RABBIT)Antibody-200-401-439 Host Rabbit TargetSpecies Yeast KnownCrossReactivity yeast Clonality Polyclonal Application PhysicalState Lyophilized ShippingCondition Ambient Format IgG Label Unconjugated Concentration 5.0mg/mLbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 ReconstitutionVolume 100µL ReconstitutionBuffer Restorewithdeionizedwater(orequivalent) Preservative 0.01%(w/v)SodiumAzide StABIlizer None GeneName APG8 RelevantLinks UniProtKB-P38182 NCBI-6319393 GeneID-852200 |
APG8AntibodyDescription
Background
Anti-ATG8antibodyisanUbiquitin-likeprotein(UBL)antibody.UBLproteinsandantibodiesfallintotwoclasses:thefirstclass,ubiquitin-likemodifiers(UBLs)functionasmodifiersinamanneranalogoustothatofubiquitin. ExamplesofUBLsareSUMO,Rub1(alsocalledNedd8),Apg8andApg12. Proteinsofthesecondclassincludeparkin,RAD23andDSK2,aredesignatedubiquitin-domainproteins(UDPs). Theseproteinscontaindomainsthatarerelatedtoubiquitinbutareotherwiseunrelatedtoeachother. IncontrasttoUBLs,UDPsarenotconjugatedtootherproteins. Apg8isrequiredforautophagy(intracellularbulkproteindegradation)inyeast. Starvedyeastcellstakeuptheirowncytoplasmintovacuolesthroughautophagicbodies. Autophagicbodiesformadouble-membranedstructurecalledtheautophagosome,whichsubsequentlyfuseswiththevacuole/lysosome.Thisprocesssimilarinmammals. Twosetsofgenes,APGandAUT,havebeenidentifiedwiththisprocess,andareresponsIBLefortwoubiquitin-likesystemsApg12andApg8,respectively. Apg12issynthesizedinitsmatureformandseemstohaveonetarget,Apg5. AlmostallApg12moleculesareconjugatedwithApg5. Aut2/Apg4processestheApg8/Aut7systematitscarboxy-terminalregion. Apg8existsintwoforms,oneismembraneboundthroughaphospholipid.Lipidation/activationofApg8ismediatedbyApg7andtransferredtoApg3andfinallyformsaconjugatewithphosphatidyl-ethanolamine(PE).Apg4cleavesApg8–PE,releasingApg8frommembrane. MorphologicalstudiesshowthatApg8localizesonthemembraneofintermediatestructuresoftheautophagosome;thistransientassociationseemstobeessentialforformationoftheautophagosome.BothApg12andApg8arehighlyconserved,withapparenthomologuesintheworm,mammalsandplants.Inhighereukaryotes,Apg8consistsofamultigenefamily.UsefulinCellBIOLOGy,MicrobiologyandSignalTransductionresearch.
Synonyms
rabbitanti-APG8Antibody,APG-8,APG8,ATG-8,ATG8,anti-ATG8Antibody,Autophagy-relatedprotein8,Autophagy-relatedubiquitin-likemodifierATG8,Cytoplasmtovacuoletargetingprotein5,AUT7,CVT5
Immunogen
ThispurifiedantibodywaspreparedfromrabbitserumafterrepeatedimmunizationswithrecombinantyeastAPG8protein.
ImmunogenType
RecombinantProtein
StorageCondition
Storeantibodyat4°Cpriortorestoration. Forextendedstoragealiquotcontentsandfreezeat-20°Corbelow. Avoidcyclesoffreezingandthawing. Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature. Thisproductisstableforseveralweeksat4°Casanundilutedliquid. Diluteonlypriortoimmediateuse.
ApplicationNote
ThispurifiedpolyclonalantibodyreactswithyeastAPG8bywesternblotandELISA.Althoughnottested,thisantibodyislikelyfunctionalinimmunohistochemistryandimmunoprecipitation. Thisantibodyusingthespecifiedconditionsmayrecognizeotherprominentintrinsicbands(UBLsortheirconjugates). OtherintrinsicbandsarereADIlydetectableinyeastlysatesatlowerantibodydilutions.Forimmunoblottinga14kDabandcorrespondingtoyeastAPG8isdetected. Mostyeastcelllysatescanbeusedasapositivecontrolwithoutinductionorstimulation.
Purity/Specifity
Anti-ATG8antibodyisalsoknownasAnti-APG8andisanIgGfractionantibodypurifiedfrommonospecificantiserumbyamulti-stepprocesswhichincludesdelipidation,saltfractionationandionexchangechromatographyfollowedbyextensivedialysisagainstthebufferstatedabove. Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-RabbitSerum.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
