品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
APC6phosphoT580AntibodyProperties Description Anti-APC6pT580(RABBIT)Antibody-600-401-876 Host Rabbit TargetSpecies Human KnownCrossReactivity human Clonality Polyclonal Application ELISA:1:1,000-1:10,000 IFMicroscopy:UserOptimized WesternBlot:1:500-1:3,000 OtherDilution:UserOptimized PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format IgG Label Unconjugated Concentration 0.97mg/mLbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 Preservative 0.01%(w/v)SodiumAzide StABIlizer None GeneName CDC16 RelevantLinks NCBI-37537763 UniProtKB-Q13042 GeneID-8881 |
APC6phosphoT580AntibodyDescription
Background
APC6(alsoknownasCelldivisioncycleprotein16homolog(CDC16Hs),anaphasepromotingcomplexsubunit6,andcyclosomesubunit6)isacomponentoftheanaphasepromotingcomplex/cyclosome(APC/C),acellcycle-regulatedubiquitinligasethatcontrolsprogressionthroughmitosisandtheG1phaseofthecellcycle. TheAPC/Ciscomposedofatleast11subunits. APC6interactswithPPP5CandCDC20.ThisproteincolocalizeswithCDC27tothecentrosomeatallstagesofthecellcycleandtothemitoticspindle. Splicevariantshavebeendescribedforthisprotein.
Synonyms
rabbitanti-APC6pT580Antibody,APC6,APC-6,Anaphasepromotingcomplexsubunit6antibody,CDC16Hsantibody,Celldivisioncycle16homologantibody,Celldivisioncycleprotein16homologantibody,Cyclosomesubunit6antibody
Immunogen
Thisaffinitypurifiedantibodywaspreparedfromwholerabbitserumproducedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoaminoacids575-588ofHumanApc6protein.
ImmunogenType
Peptide
PostTranslationalModification
Phosphorylation
StorageCondition
Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
ThisaffinitypurifiedantibodyhasbeentestedforuseinELISAandwesternblot. Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Expectaband~72kDa,71.5kDaor65.9kDainsizecorrespondingtoisoformsofAPC6bywesternblottingintheappropriatecelllysateorextract.
Purity/Specifity
Thisproductisanaffinitypurifiedantibodyproducedbyimmunoaffinitychromatographyusingphosphopeptidecoupledtoagarosebeadsfollowedbysolidphaseadsorption(s)againstnon-phosphopeptideandnon-specificpeptidetoremoveanyunwantedreactivities.ThisantibodyisspecificforphosphorylatedhumanAPC6proteinatthepT580residue.BLASTanalysisindicates100%homologyoftheimmunizingsequencewithAPC6fromhuman. Nocrossreactivityisexpectedagainsthomologuesfromothersources. Thisantibodyisexpectedtoreactwithallisoformsofthisprotein. Minimalreactivityisexpectedwiththenon-phosphorylatedformoftheprotein.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
