品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Apc11AntibodyProperties Description Anti-APC11(C-terminalspecific)(RABBIT)Antibody-100-401-A15 Host Rabbit TargetSpecies Human KnownCrossReactivity human Clonality Polyclonal Application ELISA:1:2,000-1:10,000 WesternBlot:1:500-1:1,000 Immunohistochemistry:UserOptimized OtherDilution:UserOptimized PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format Antiserum Label Unconjugated Concentration 85mg/mLbyRefractometry Buffer None Preservative 0.01%(w/v)SodiumAzide StABIlizer None GeneName ANAPC11 RelevantLinks UniProtKB-Q9NYG5 NCBI-19924286 GeneID-51529 |
Apc11AntibodyDescription
Background
APC11isalsoknownasAnaphasepromotingcomplexsubunit11,APC11,Cyclosomesubunit11,HepatocellularcarcinomaassociatedRINGfingerprotein,andHSPC214. APC11isacomponentoftheanaphasepromotingcomplex/cyclosome(APC/C),acellcycle-regulatedE3ubiquitinligasethatcontrolsprogressionthroughmitosisandtheG1phaseofthecellcycle.APC11mayfunctiontorecruittheE2ubiquitin-conjugatingenzymestothecomplex. APC11interactswiththecullindomainofANAPC2andalsointeractswithUBE2D2. APC11showsbothacytoplasmicandnuclearlocalization. APC11isexpressedathighlevelsinskeletalmuscleandheart;inmoderatelevelsinbrain,kidney,andliver;andatlowlevelsincolon,thymus,spleen,smallintestine,placenta,lungandperipheralbloodleukocyte. APC11isamemberoftheRING-typezincfingerfamilyandisauto-ubiquitinylated.
Synonyms
rabbitanti-Apc11Antibody,Anaphasepromotingcomplexsubunit11homologantibody,APC11anaphasepromotingcomplexsubunit11homologantibody,Cyclosomesubunit11antibody,HepatocellularcarcinomaassociatedRINGfingerproteinantibody,HSPC214antibody,Apc
Immunogen
Thisantibodywaspreparedfromwholerabbitserumproducedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoaminoacids76-84ofHumanAPC11(C-terminal)coupledtoKLH.
ImmunogenType
Peptide
StorageCondition
Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
ThisantibodyreactswithhumanAPC11bywesternblotandimmunoprecipitation. Theantibodyimmunoprecipitatesinvitrotranslatedproteinandproteinfromoverexpressingcelllysates(usingHeLaandNIH-3T3,andothers). Coimmunoprecipitationofrelatedproteins(APC2)doesoccur. A9.8kDabandcorrespondingtohumanAPC11isdetected.MostcelllinesortissuesexpressingAPC11canbeusedasapositivecontrol. Researchersshoulddetermineoptimaltitersforotherapplications.
Purity/Specifity
Thisproductismonospecificantiserumprocessedbydelipidationanddefibrinationfollowedbysterilefiltration.ThisproductreactswithhumanandmouseAPC11.CrossreactivitymayalsooccurwithAPC11fromothersources. SufficientsequencedifferencesexisttosuggestthatthisantibodywouldnotreactwithotherRINGboxproteinssuchasROC1andROC2.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
