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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
Anti-ElastaseAntibodyBiotinConjugatedPropertiesDescription Anti-ELASTASE(RABBIT)AntibodyBiotinConjugated-200-4668-0100 Host Rabbit TargetSpecies Swine KnownCrossReactivity swine Clonality Polyclonal Application PhysicalState Lyophilized ShippingCondition Ambient Format IgG Label Biotin Concentration 1.0mg/mLbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 ReconstitutionVolume 100µL ReconstitutionBuffer Restorewithdeionizedwater(orequivalent) Preservative 0.01%(w/v)SodiumAzide StABIlizer 10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree GeneName CELA1 RelevantLinks UniProtKB-P00772 NCBI-P00772.1 GeneID-396766 |
Anti-ElastaseAntibodyBiotinConjugatedDescription
Background
Anti-ElastaseantibodydetectstheElastaseprotein.Elastasemetabolizeselastin,anelasticfiber,whichtogetherwithcollagen,determinesthemechanicalpropertiesofconnectivetissue.TheneutrophilformbreaksdowntheOutermembraneproteinA(OmpA)ofE.coliandotherGram-negativebacteria.ElastasealsohastheimportantimmunologicalroleofbreakingdownShigellavirulencefactors.Thisisaccomplishedthroughthecleavageofpeptidebondsinthetargetproteins.Thespecificpeptidebondscleavedarethoseonthecarboxysideofsmall,hydrophobicaminoacidssuchasglycine,alanine,andvaline.Thisantibodyissuitableforresearchersinvestigatingbiochemistryandstemcells.
Synonyms
rabbitanti-ElastaseAntibodybiotinConjugation,biotinConjugatedrabbitanti-ElastaseAntibody,Fecalpancreaticelastase1antibody,HLEantibody,Medullasinantibody,PancreaticelastaseIIAantibody,PE1antibody,PE1antibody,ProteaseEantibody
Immunogen
Elastase[PorcinePancreas]
ImmunogenType
NativeProtein
StorageCondition
Storevialat4°Cpriortorestoration.Forextendedstoragealiquotcontentsandfreezeat-20°Corbelow.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
Anti-ElastaseAntibodyhasbeenassayedagainst1.0ugofElastaseinastandardcaptureELISAusingPeroxidaseConjugatedStreptavidin#S000-03andABTS(2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid])code#ABTS-100asasubstratefor30minutesatroomtemperature. Aworkingdilutionof1:4,000to1:20,000ofthereconstitutionconcentrationissuggestedforAnti-ElastaseAntibody
Purity/Specifity
Anti-Elastase(Rabbit)AntibodyBiotinConjugatedisanIgGfractionantibodypurifiedfrommonospecificantiserumbyamulti-stepprocesswhichincludesdelipidation,saltfractionationandionexchangechromatographyfollowedbyextensivedialysisagainstthebufferstatedabove. Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-Biotin,anti-RabbitSerumaswellaspurifiedandpartiallypurifiedElastase[PorcinePancreas]. CrossreactivitiesagainstElastasefromothersourcesmayoccurbuthavenotbeenspecificallydetermined.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.