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苏州工业园区生物纳米园A4#216
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商品描述
Angiopoietin2AntibodyProperties Description Anti-Angiopoietin2(RABBIT)Antibody-100-401-402 Host Rabbit TargetSpecies Mouse KnownCrossReactivity human,mouse Clonality Polyclonal Application ELISA:1:5,000-1:25,000 WesternBlot:1:500-1:2,000 Immunohistochemistry:1:200-1:500 OtherDilution:UserOptimized PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format Antiserum Label Unconjugated Concentration 80mg/mLbyRefractometry Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 Preservative 0.1%(w/v)SodiumAzide StABIlizer None GeneName ANG-2 RelevantLinks UniProtKB-O35608 NCBI-O35608.2 GeneID-11601 |
Angiopoietin2AntibodyDescription
Background
AntiAntiopoietin-2AntibodyrecognizesAngiopoietin-2(Ang-2)thathasimportanceindevelopmentoftheendotheliumthroughregulationoftyrosinephosphorylationofthemembranereceptorTie-2/Tek.Ang-1bindingtoTie-2/Tekcausesphosphorylationofthereceptor.Ang-2competesforthisbindingandthusblocksreceptorphosphorylation. Ang-2expressionoccursatsitesofvascularremodelling:dorsalaortaandmajoraorticbranches,ovary,placentaanduterus.Thisantibodyissuitableforcardiovascularresearch.
Synonyms
rabbitanti-Angiopoietin2Antibody,rabbitanti-ANG2antibody,AGPT2antibody,Agpt2antibody,ANG2antibody,ANG2antibody,Angiopoietin2aantibody,Angiopoietin2Bantibody,Angiopoietin2antibody,ANGPT2antibody,ANGPT2antibody,Tie2ligandantibody
Immunogen
Thiswholerabbitserumwaspreparedbyrepeatedimmunizationswithasyntheticpeptide,correspondingtoaregionneartheN-terminusofmouseangiopoietin-2protein,conjugatedtoKLHusingmaleimide.
ImmunogenType
Peptide
StorageCondition
Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
Angiopoietin2Antibodyissuitableforwesternblottingandotherantibodybasedassays. A1:500dilutionisrecommendedforwesternblotting. Thereactionofthisantiserumdirectlywithcellsupernatantsmayresultinhighbackgroundduetoreactivityofcomponentsintheserum. Thiscanbealleviatedbyfirstimmunoprecipitatingtheantibody:antigencomplexandthendetectingtheantigen.Thismethodresultsinaveryclean,strongsignal.
Purity/Specifity
Angiopoietin2Antibodyantiserumisdirectedagainstmouseangiopoietin-2andshowsnoreactivitywithangiopoietin-1frommouse-derivedproteins. A75kDabandisdetectedwhenassayedbyimmunoblot. Thisreagentcross-reactswithhumanangiopoietin-2. Partiallycross-reactivityisnotedwithhumanangiopoietin-1.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
