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蚂蚁淘在线 / 品牌中心 / Pluriselect / Pluriselect/Lympho 24+ Spin Medium / PBMC 24+ Spin Medium/60-00093-12/

Pluriselect/Lympho 24+ Spin Medium / PBMC 24+ Spin Medium/60-00093-12/

价格
￥480.00
货号：60-00093-12
浏览量：127
品牌：Pluriselect
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Details:

Lympho24+SpinMediumisareadytouse,sterilemediumforpreparationofPeripheralBloodMononuclearCell(PBMC)from12-48houroldperipheralblood,buffycoat,bonemarrow,orcordbloodbyasimplecentrifugationprocedure.ItreducestheRBCcontaminationbutalsoreducesPBMCyieldby20%.

MostdensitygrADIentmediaarelimitedfortheuseoffreshwholeblood.Lympho24+SpinMediumisrecommendedfortheuseofwholebloodthatisolderthan8hours.Thishelpstoreducethetimedependedcontaminationwithgranulocytes.

Theenrichedmononuclearcellscanbeusedforawiderangeofdownstreamapplicationssuchasmagneticactivatedcellsorting(MACS1)orstemcellresearch.

SeparationschemeforPBMCenrichmentwithLympho24+SpinMedium

AtfirstcarefullylayerthesamplematerialontopoftheLympho24+SpinMedium.Avoidamixingofthetwophases.Alternativelyit’spossIBLetousepluriMatetubes.Thebarrierpreventsamixingofsamplematerialanddensitysolution.FurThermorethetubeenablespouringofftheenrichedcellsafterthefirstcentrifugationstep.Afterthedensitycentrifugationaspiratetheupperlayer(Plasmaanddilutionbuffer).Afterwardstransferthemononuclearcelllayertoanewconicaltubeandwashthecells.


PBMC preparation with Lympho 24+ Spin Medium. The sample material will be overlayed on top of the density medium.

Fig.1:PreparationofPBMCwithLympho24+SpinMedium.Thesamplematerialwillbeoverlayedontopofdensitygradientmedium.

Whole blood after density gradient centrifugation with Lympho 24+ Spin. The PBMC can be found on top of the Lympho 24+ Spin Medium.

Fig.2.:Layersafterdensitygradientcentrifugation.ThePBMCcanbefoundontopoftheLymphoSpinMedium.Erythrocytes,Granulocytesanddeadcellswillpassthemediumandcanbefoundatthebottomofthetube.


1MACSisatrademarkofMiltenyiBiotecGmbH

MoreInformation:

SampleMaterialWholeBlood,PBMC,BuffyCoat,CordBlood,BoneMarrow,PrimaryCellSolution
StorageConditionRoomTemperature
RegulatoryStatementForresearchuseonly.Notforuseindiagnosticprocedures,
Pluriselect-BSA储备溶液（50毫升）$ 38.00细节1x PBS中的10％BSA储备溶液可用于细胞培养，细胞分离洗涤缓冲液，蛋白质印迹法中的封闭步骤，ELISA，IHC和核酸检测方法。Blocker BSA的功能即用型-无需溶解粉末纯-高质量的牛血清白蛋白含量为10％的溶液与其他组分的交叉反应机会更少方便-针对特定应用的最佳阻塞效果更多信息更多信息尺寸50毫升不育性不育pH范围pH值7.4运输条件室内温度储存情况4-8°摄氏度监管声明仅供研究使用。不用于诊断过程，