Details:
LeukoSpinMediumisareadytouse,steriledensitygrADIentmediumfortheisolationofLeukocytesinhighyieldfromfreshperipheralbloodandbuffycoatbyasimplecentrifugationprocedure.
Mononuclearandpolymorphonuclear(multi-lobednuclei)cellscanbeenrichedbyasimpledensitygradientcentrifugationwithLeukoSpinMedium.TheenrichedLeukocytescanbeusedforawiderangeofdownstreamapplicationssuchasmagneticactivatedcellsorting(MACS1)orstemcellresearch.
SeparationschemeforLeukocyteenrichmentwithLeukoSpinMedium
AtfirstcarefullylayerthesamplematerialontopoftheLeukoSpinMedium.Avoidamixingofthetwophases.Alternativelyit’spossIBLetousepluriMatetubes.Thebarrierpreventsthemixingofthetwophasesandenablestopourofftheenrichedcellsafterthefirstcentrifugationstep.Afterthedensitycentrifugationstepaspiratetheupperlayer(Plasmaanddilutionbuffer).Thentransferthemononuclearandthepolymorphonuclearcelllayertoanewconicaltubeandwashthecells.
Fig.1:IsolationofallLeukocyteswithLeukoSpindensityseparationmedium.
Fig.2:Enrichedperipheralbloodmononuclearandpolymorphonuclearcells.
MoreInformation:
| SampleMaterial | WholeBlood,PBMC,BuffyCoat,CordBlood,BoneMarrow,PrimaryCellSolution |
|---|---|
| StorageCondition | RoomTemperature |
| RegulatoryStatement | Forresearchuseonly.Notforuseindiagnosticprocedures, |
