TheTransforMax™EC100D™pir⁺ElectrocompetentE.coliandTransforMax™EC100D™pir-116ElectrocompetentE.colieachconstitutivelyexpresstheπprotein(thepirgeneproduct)forreplicationofplasmidscontainingtheR6Kγoriginofreplication(R6Kγori).ThecellsarederivedfromEpicentre'sTransforMax™EC100™ElectrocompetentE.colibyP1phagetransductionwithastraincontainingthepir⁺orpir-116genelinkedtoadihydrofolatereductase(DHFR)Marker.

TheTransforMaxEC100Dpir⁺cellswillmaintainR6Kγoricontainingplasmidsatapproximately15copiespercell,¹forcloningofpotentiallytoxicorunstableDNAsequences.TheTransforMaxEC100Dpir-116cellsareforhigh-copypropagationofupto250rescueplasmidcopiespercell.¹

Benefits

• AcceptslargeclonesforunbiasedpropagationandstABIlityoflargerescueclones.
• lacZΔM15forblue/whitescreeningofrecombinants.
• Restrictionminus[mcrA,Δ(mrr-hsdRMS-mcrBC)]enablesefficientcloningofmethylatedDNA.
• Endonucleaseminus(endA1)toensurehighyieldsofDNA.
• Recombinationminus(recA1)forgreaterstabilityoflargeclonedinserts.

Genotypes

TransforMaxEC100Dpir⁺ElectrocompetentE.coli:
F^-mcrAΔ(mrr-hsdRMS-mcrBC)φ80dlacZΔM15ΔlacX74recA1endA1araD139Δ(ara,leu)7697galUgalKλ-rpsL(Str^R)nupGpir⁺(DHFR)

TransforMaxEC100Dpir-116ElectrocompetentE.coli:
F^-mcrAΔ(mrr-hsdRMS-mcrBC)φ80dlacZΔM15ΔlacX74recA1endA1araD139Δ(ara,leu)7697galUgalKλ-rpsL(Str^R)nupGpir-116(DHFR)

TransformationEfficiency
Greaterthan5×10⁹colonyformingunits(cfu)/µgwithpR6KancontrolDNA.

References

1. Metcalf,W.W.etal.(1994)Gene138,1.

ORDERINFORMATION

Eachproductcontains5tubeseachcontaining100µLofelectrocompetentcells,whichisenoughcellsfor10electroporations.AcontrolvectorcontainingandR6Kγoriisalsoincludedwitheachproduct. Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限，在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应，您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量，可靠性和稳定的性能。