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商品描述
RandominsertionofT7promotertodrivegeneexpressionwithT7RNApolymerase
- InsertT7promoterrandomlyintoanyDNAsequenceinvitro
- IsolatepositivecloneswiththeselectablekanamycinMarker
- Expressresultingproductsinvivoorinvitro
- MinimizeinsertionbiaswiththehyperactiveTn5system,knownforhighestlevelofrandomness
Applications
- RandominsertionofasingleT7RNAPolymerasepromotertosynthesizeRNAfromanyregionofclonedDNA.
TheEZ-Tn5™<T7/KAN-2>PromoterInsertionKit*providesaneasyandreliablemethodtorandomlyinsertatransposoncontainingaphageT7RNApolymerasepromoterintoanyDNAmoleculeinvitro.Thetransposonendhasnoterminationsequences,soRNAcanbeproducedfromchoseninsertionclonesbyinvitrotranscriptionfromtheT7RNApolymerasepromoterusing,forexample,anyofEpicentre'sT7RNAPolymeraseTranscriptionKits.RNAcanalsobegeneratedforinvivoexpressionstudiesincellshavinganinducIBLeT7RNApolymerasegene.
 | Figure1(clicktoenlarge).RNAtranscriptscanbeproducedfrominsertionclonesinvitroorinvivousingtheEZ-Tn5™<T7/KAN-2>PromoterInsertionKit. |
*Coveredbyissuedand/orpendingpatents,exclusivelylicensedorassignedtoEpicentre®(anIllumina®Company).
