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蚂蚁淘在线 / 品牌中心 / Lucigen / Lucigen/MessageBOOSTER™ cDNA Synthesis Kit for qPCR/MB060124/24 Reactions

Lucigen/MessageBOOSTER™ cDNA Synthesis Kit for qPCR/MB060124/24 Reactions

价格
￥10520.00
货号：MB060124
浏览量：127
品牌：Lucigen
服务
全国联保
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正规发票
签订合同
商品描述

ProduceamplifiedamountsofCDNAfromprecious,limitingtotalRNAsampleswithoutintroducingbias.

  • PerformRNAamplificationonpurifiedtotalRNAfollowedbycDNAsynthesisanddetectionFormerly from Epicentre
  • Amplifywiththishigh-fidelity,linearRNAamplificationprocessthatpreservestherelativetranscriptabundanceofthesample.
  • Getmoredataoutofprecioussamples–uselessRNAformoreRT-qPCRreactions
  • ReADIlyandreproducIBLydetectevenlow-abundancetranscriptsinRNAfromasinglecell(CTvalues<35cycles)
  • CollectsmallRNAsampleslessoften.
  • FastprotocolamplifiesandsynthesizescDNAinonly1day.
Applications
  • SignificantlyincreasingthenumberofqRT-PCRsthatcanbeobtainedfromverysmallamountsoftotalRNA,aslittleasonecell(10pg).
  • GenerationoflargeamountsofcDNAfromverysmallsamplesofintacttotalRNAforarchivalpurposes.

TheMessageBOOSTER™cDNASynthesisKitforqPCRenablestheusertoperformsensitiveqPCRamplificationsusingthetotalRNAfromverysmallpopulationsofcells,evenfromaslittleasonecell(Table1).

ThekitamplifiesthemRNAcontainedinasmalltotalRNAsampleandthenconvertstheamplifiedRNAtocDNAthatisreadyforPCRorqPCR(Fig.1).TheMessageBOOSTERcDNASynthesisKitforqPCRusesoligo(dT)toprimecDNAsynthesis.Therefore,thiskitisbestusedwithintacttotalRNApreparations.

Figure1(clicktoenlarge).OverviewoftheMessageBOOSTER™cDNASynthesisKitprocedure.ThekitusesalinearRNAamplificationprocesstoamplifytheRNAfromasmallpopulationofcells,thenconvertstheamplifiedRNAtocDNAthatisreadyforPCR.Theprocedurecanbecompletedin1day.

Figure2.SensitivityoftheMessageBOOSTER™cDNASynthesisKit.cDNAproducedfrom10pgoftotalNRKRNAsignificantlyimprovedthesensitivity(loweredtheCT)ofdetectingthelow-abundancePBGDtranscriptcomparedtocDNAproducedfrom10pgofunamplifiedRNA.

AmountofTotalRNAinaMessageBOOSTER™ReactionNumberofqPCRsThatCanBePerformed
Low-toMedium-AbundanceTranscriptsaMedium-toHigh-AbundanceTranscriptsa
10pg(~1cell)≥10≥100
100pg(~10cells)≥100≥1,000
500pg(~50cells)≥500-1,000≥5,000-10,000
Table1.ThenumberofqPCRamplificationsthatcanbeperformedusingcDNAproducedbyaMessageBOOSTER™cDNASynthesisKit.ThenumberofqPCRsisdependentontheamountofinputtotalRNAandtheabundanceofthetranscript(s)ofinterest.

a.Low-AbundanceTranscripts=1-1,000copiespercell
Medium-AbundanceTranscripts=1,000-10,000copiespercell
High-AbundanceTranscripts=10,000-100,000copiespercell

ProductCitations

  1. Grunenwald,H.etal.(2006)EpicentreForum13(3),22.
  2. DeFazio,R.A.etal.(2006)J.Neuroscience26,3971.
  3. Kaeffer,B.etal.(2007)PediatricResearch62,564.
  4. Lochner,M.,etal.(2008)J.Exp.Med.205,1381.
  5. Graham,D.M.etal.(2008)J.Neurophysiology,99,2522.
  6. Liu,X.et.al.(2009)Am.J.Physiol.LungCellMolPhysiol.296,L158
  7. Yakovlev,I.A.etal.(2008)FungalGeneticandBIOLOGy45,498
  8. Jung,Y.etal.(2008)StemCellsExpress10,1634
  9. Xi,D.etal.(2008)J.NeurosciMethod10,1016
  10. Michel,M-L.etal.(2008)Proc.Nat'lAcadSci-USA105(50),19845
  11. Satoh-Takayama,N.etal.(2008)Immunity29,958
  12. Bouskra,D.etal.(2008)Nature10,1038
  13. Yakovlev,I.A.etal.(2008)FungalGeneticsandBiology45,498
  14. Roberts,C.D.et.al.(2009)J.Physiology587,1657
  15. Peduto,L.et.al.(2009)J.Immunology182,5789
  16. Xi,D.et.al.(2009)Int'lJ.Neuropsychopharmacology,May13:1-14

ORDERINFORMATION

MessageBOOSTER™T7-Oligo(dT)PrimerA,RNaseInhibitor,MessageBOOSTER™ReverseTranscriptionPreMix,MessageBOOSTER™DNAPolymerasePreMix1,MessageBOOSTER™DNAPolymerase1,MessageBOOSTER™cDNAFinishingSolution,MessageBOOSTER™RandomPrimers,MessageBOOSTER™RNaseH,MMLVReverseTranscriptase,MessageBOOSTER™InVitroTranscriptionPreMixA,MessageBOOSTER™T7RNAPolymerase,MessageBOOSTER™T7TranscriptionBuffer,RNase-FreeDNaseI,ForwardandReverseControlPCRPrimers,DTT,RNase-FreeWater,Poly(I),NRKTotalRNAControl. Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限，在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应，您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量，可靠性和稳定的性能。