- Powerfulnativereversetranscriptaseandstranddisplacementactivitiesallow gene-specificLAMPandRT-LAMPatupto72°C
- Amplifyfromanynucleicacidtemplate:RNA,DNAandCDNA
- FasterthanBstExoMinus
- Completekitwithcontrols,MagnesiumSulfateandBetaineforeasyoptimization
Theworld"sonlyenzymeforRNALAMPandDNALAMP
TheOmniAmp® polymeraseistheonlyenzymecapableofLoop-mediatedIsothermalAmplification(LAMP)frombothRNAandDNAtargets.Theenzymehasatemperatureoptimumof72°C,allowingincreasedspecificityandflexibilitycomparedtoBstExoMinusortwo-stepprocesses.ThenativereversetranscriptaseactivityofthisenzymemeansyoudonotneedtoaddaseparatereversetranscriptaseforRNAtargets.
Flexibility:Theenzymecomeswith10XOmniAmpBuffer,MagnesiumSulfate,andBetaine.Thebufferisdesignedtoallowforawiderangeofadditivesandeasyoptimizationfollowingtheinstructionsprovidedintheusermanual.
ReliABIlity:Achievefasteramplificationathighertemperatureswithoutsacrificingsensitivity.Theenzymehashightargetspecificityandlowbackground.Everybatchisqualitytestedtoensurenounwantednucleaseactivitywillinterferewithyoursensitiveapplications.
Primerdesign:ArobustLAMPreactiondependsonproperdesignoftheamplificationprimers.PleasefollowthisPDFguidetoproperlyusethePrimerExplorersoftware.Theuseofsixprimersisoptimal;reactionsaresignificantlysloweranddetectionislesssensitiveifonlyfourprimersareused.
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| Figure1.QuantitationofLAMPAmplificationfromaDNATarget Triplicate25µLLAMPreactionsweresetupusingaprimersetforaS.aureusClfAtargetgeneandincludedvaryingtargetDNAdilutionsasindicatedplusaNoTargetControl(NTC).Reactionsweresetupasrecommendedbyeachmanufacturer.OmniAmp®Reactions: 1XReactionBufferplus4mMMgSO4(10mMtotal),0.8mMdNTPs,150mMbetaine,0.5Xprimers,1XFionaGreenand0.5XOmniAmpPolymerase.Bst(NEB)Reactions:1XReactionBuffer,6mMMgSO4total,0.8mMdNTPs,1XFionaGreen,0.5Xprimersand8UBstPolymerase. OmniAmpreactionswereincubatedat68°CandBstreactionsat65°CinaBio-RadCFX96™Real-Timeinstrument(separateruns)andfluorescencewasmonitoredover60minutes.Averagefluorescenceandstandarddeviationsforeachsetoftriplicatereactionsareplotted. |
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| Figure2.QuantitationofLAMPAmplificationfromaRNATarget. Triplicate25µLLAMPreactionsweresetupusingaprimersetforthePositiveControl(MS2RNA)includedwiththeOmniAMP®Kitand1µLofTargetRNAdilutedasindicatedplusaNoTargetControl(NTC).Reactionsweresetupasrecommendedbyeachmanufacturer.OmniAmpReactions: 1XReactionBufferplus4mMMgSO4(10mMtotal),0.8mMdNTPs,150mMbetaine,1Xprimers,1XFionaGreenand1XOmniAmpPolymerase.Bst3.0(NEB)Reactions:1XReactionBuffer,6mMMgSO4total,1.4mMdNTPs,1XFionaGreen,1Xprimersand8UBst3.0Polymerase. OmniAmpreactionswereincubatedat70°CandBst3.0reactionsat65°CinaBio-RadCFX96™Real-Timeinstrument(separateruns)andfluorescencewasmonitoredover60minutes.Averagefluorescenceandstandarddeviationsforeachsetoftriplicatereactionsareplotted. |
Licensinginformation:LucigenisafullylicensedproviderofLAMPreagentsforresearchuse.PatentsWO00/28082,WO01/34790,andWO01/77317regardingtheLAMPmethodareownedbytheEikenChemicalCo.Ltd.OmniAmp™andBstPolymerase,ExonucleaseminusaresoldbyLucigenunderlicenseforuseinLAMPforresearchuseonly.TheproductsmaynotbeusedforLAMP-basedhumanordiagnosticpurposeswithoutobtainingalicensefromEiken.USPatent8093030forOmniAmpDNAPolymeraseisownedbyLucigenCorp.
Itisthesoleresponsibilityofthebuyertoensurethatuseoftheproductdoesnotinfringethepatentrightsofthirdparties.Ifthepurchaserisnotwillingtoaccepttheseuselimitations,LucigenCorporationiswillingtoacceptreturnoftheproductforafullrefund.
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