- GreatTaqPolymeraseperformanceatalowprice.
- Choiceofreactionbuffers,withorwithoutMgCl2.
- Non-proofreADIngPolymerase
FreeSampleAvailable
At$96for1000unitslistprice(quantitydiscountsavailable),EconoTaq’slowpriceiscoupledwithhighqualityandperformance.
QCspecificationsforEconoTaqarerigorous:
- Greaterthan99%purebySDSgelelectrophoresis(seeFigure1).
- NodetectableDNAcontaminationasdeterminedbyPCRusinggenericprimers.
- Nodetectableendonuclease(nicking)activity.Incubationof10UofEconoTaqDNAPolymerasewith1µgofsupercoiledpBR322DNAfor16hoursat70°Cresultsinnodetectableconversiontorelaxedorlinearformsbyagarosegelelectrophoresis.
- Nodetectableexonucleaseactivity.Incubationof10UofEconoTaqDNAPolymerasewith1µgofHindIII-cutlamBDaDNAfor16hoursat70°Cresultsinnosmearingofbandsonagarosegels.
EconoTaqPerformance
AsshowninFigures2and3Lucigen’sEconoTaqDNAPolymeraseperformsaswellas,orbetterthan,moreexpensiveTaqpreparationsfromseveralothersuppliersinroutinePCR.EconoTaqisaseffectiveinDNAamplificationasanotherstandardPCRenzyme,TflDNApolymerase(Figure4).Inthiscase,thebackgroundofnon-specificamplificationwasmuchlowerwithEconoTaq(compare“+”and“–“lanesforEconoTaqandTflinFigure4).EconoTaqDNAPolymerasealsooffershighlot-to-lotreproducibilityandreliABIlity(Figures2and4)
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Figure1.HighpurityofEconoTaqDNAPolymerase(SDSPAGE).Lane1,broadrangemolecularweightMarkers;Lane2,LucigenEconoTaqDNAPolymerase | Figure2.TaqDNApolymerasefromPromegaandNewEnglandBiolabswerecomparedtoLucigen’sEconoTaqDNAPolymerase(2differentlots)inamplifyingtheampicillingene(0.8kb)inapUC19vector.(–),noDNA.(+),DNAadded(40ng).MW,1kbladder. |
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Figure3.EconoTaqvs.AmpliTaq®(AppliedBiosystems)DNApolymeraseingenotyping.AllPCRreactionswereperformedinaRoboCycler96(Stratagene).Hip1genotypingwasperformedusingthefollowingPCRconditions:94°Cfor1min,35cyclesof94°Cfor30sec,62°Cfor60sec,72°Cfor90sec,and72°Cfor7min.Shh,CdoandGas1genotypingwereperformedusingthefollowingPCRconditions:94°Cfor2min,35cyclesof94°Cfor60sec,65°Cfor60sec,72°Cfor90sec,and72°Cfor7min.AllPCRreactionscontainedfinalconcentrationsof1µMofeachprimer,200µMdNTPs,1Xcresolredloadingdye,and1UoftheindicatedTaqpolymerase.SequencesforallPCRprimershavebeenpreviouslypublished(referencesavailable). |
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Figure4.PCRamplificationwasperformedunderstandardconditionsusingthreedifferentlotsofEconoTaqDNAPolymeraseandbuffer,orduplicatereactionswithTflDNApolymeraseandbuffer(Promega).Reactionscontainedprimersspecificforthe16SribosomalRNAgene,withBacillusgenomicDNA(+)ornoDNA(-)asatemplate(1450bpproductexpected). |
PleaseNote:
SomeapplicationsinwhichLucigen'sEconoTaqDNAPolymerasecanbeusedmaybecoveredbypatentsissuedandapplicableintheUnitedStatesandcertainothercountries.Becausepurchaseofthisproductdoesnotincludealicensetoperformanypatentedapplication,usersofthisproductmayberequiredtoobtainapatentlicensedependingupontheparticularapplicationinwhichtheproductisused.ThePCRprocessisthesubjectofEuropeanPatentNos.201,184and200,262ownedbyHoffman-LaRoche.ThosepatentsexpiredonMarch28,2006.ThecorrespondingPCRprocesspatentsintheUnitedStatesexpiredonMarch29,2005.Itisthesoleresponsibilityofthebuyertoensurethatuseoftheproductdoesnotinfringethepatentrightsofthirdparties.
