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蚂蚁淘在线 / 品牌中心 / Lucigen / Lucigen/MasterPure™ Gram Positive DNA Purification Kit/MGP04100/100 Purifications
商品描述
PurifygenomicDNAfromchallengingGrampositivebacteriaforawidevarietyofmolecularBIOLOGyapplications
Producesclean,highmolecularweightgenomicDNAreadyforPCRandothermolecularbiologyapplicationssuchasrestrictiondigestion,cloning,andSouthernblotting- Scaleablemethodforlargesamplevolumeswithnowastingofreagentswithsmallersamples
- Completekitincludeslysozyme;noseparatepurchaserequired
- Robustkitworksonavarietyoftestedspecies
- FlexIBLekitalsopurifiesgDNAfromGramnegativebacteria
TheMasterPure™GramPositiveDNAPurificationKitprovidesallofthereagentsneededtopurifyDNAfromGrampositivebacteria.ThesebacterialysemorereADIlyaftertreatmentwithReady-Lyse™LysozymeandtheGram-PositiveCellLysisSolutionincludedinthekit.Ready-LyseLysozymeisastablesolutionofarecombinantlysozymefromanonmammalian,nonaviansource.IthashighspecificactivityanddoesnotbindDNA.1,2
ExamplesofGram-positivebacteriatestedwiththiskitarelistedinTable1.ThesizerangeofDNApurifiedbytheMasterPureGramPositiveDNAPurificationKitisshowninFig.1.TheDNApurifiedusingthekitissuitableforPCRanalysis(Fig.2),andothermolecularbiologyapplications.
| Species | CultureMedium | Ready-Lyse™Incubation | DNAYieldµg/ml |
| Bacillussubtilis | Brain-HeartInfusion(BHI) | 30min | 9.0 |
| Listeria monocytogenes | BHI | Overnight | 3.3 |
| Staphylococcusaureus | BHI | Notneeded | 8.0 |
| Streptococcusmutans | Todd-Hewitt | Overnight | 3.0 |
| Lactococcuslactis | M17 | 30min | 1.1 |
Table1.IncubationtimesneededforDNArecoveryfrom1mlofGrampositivebacterialculture.
| . |
| Figure1.ElectrophoreticanalysisofDNApurifiedusingtheMasterPure™GramPositiveDNAPurificationKit.PanelA:DNApurifiedfromB.subtilis(ATCC6051)wasseparatedona1%agarosegelandstainedwithSYBR®Gold.LaneM,kilobaseladder;lane1,300ngofB.subtilisDNA.PanelB:Pulse-fieldgelelectrophoresisofB.subtilisDNA.LaneM,PhagelamBDaladder;lane1,300ngofDNA;lane2,600ngofDNA | Figure2.PCRanalysisofDNApurifiedusingtheMasterPure™GramPositiveDNAPurificationKit.B.subtilisDNAwasamplifiedbyPCRusingBacF/BacRprimers,andtheproductswereanalyzedbyagarosegelelectrophoresis.LaneM,100-bpladder;lane1,PCRproductfrom0.5ngofB.subtilisDNA;lane2,no-templatecontrol. |
References
- Hoffman, L. and Jarvis, B. (2003) Epicentre Forum 10(3), 3.
- Jarvis,B.andHoffman,L.(2004)EpicentreForum11(3),7.
- Luna,V.A.etal.(2006)J.Clin.Microbiol.44,2367.
- Li,J.etal.(2007)Immunol.75,1811.
