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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
RapidextractionofRT-PCR-readyRNAfromculturedmammaliancells
Fast:Extractend-pointandreal-timeRT-PCRreadyRNAinminutesfromlowtohighnumbersofsamples- Simple:Singletubeprotocol
- CompatIBLe:ExtractedRNAiscompatiblewithbothreal-timeandendpointRT-PCR
- Automation-friendly:Simpleprotocolmakesincorporationintoanautomatedworkfloweasy
- Flexible:ADnaseIdigestionstepcanbeaddedforsensitivedownstreamapplicationswhennecessary
- Safe:Notoxicreagentsused
TheQuickExtract™RNAExtractionKitisafast,simplewayofpreparingRNAforRT-PCR(bothend-pointandreal-time).Thesingle-tubesystemrequiresonlyvortexmixingtolysethecells,andpreparetheRNAforCDNAsynthesis.Theresultiseasyprocessingofonetohundredsofsamplesinminutes,withnosamplelossortoxicorganicsolvents.TheQuickExtractRNAExtractionSolutionworkswithculturedadherentandsUSPensioncellsincludingbuccalcells,andhasbeentestedonhuman,mouse,rat,E.coli,andS.aureuscellcultures.Itisnotsuitablefortissuesamplesorplantsamples.AnoptionalDNaseItreatmentmayimprovecertaindownstreamapplications.
Applications
- PreparationofRNAfromculturedadherentandsuspensioncellsforRT-PCR.
 | Figure1.End-pointRT-PCRofdifferentregionsofa14kbmessageusingHeLacellextractwiththeQuickExtract™RNAExtractionKit.Asamplecontaining105HeLacellswaslysedin100µLofQuickExtractRNAExtractionSolutionbyvortexmixing.ThelysatewasreversetranscribedwiththeMMLVReverseTranscriptase1stStrandcDNASynthesisKitusingstandardconditionsandrandomprimers.ThecDNAwasthenamplifiedwithsixprimersetstop532usingtheFailSafe™PCRSystem.LaneM,100 bpladder;Lane1,12984to13892;lane2,9406to10202;lane3,5194to5802;lane4,4191to4690;lane5,2280to2676;andlane6,1029to1329. |
 | Figure2.ComparativeyieldofRT-PCRproductwithdifferentRNAextractionkits.Lysateswerepreparedaccordingtomanufacturers'instructionsandusedastemplatetoproducecDNAusingtheMMLVRT1stStrandcDNASynthesisKit,followedbyPCRusingtheFailSafe™PCRSystemwithprimersfortheALDOAgene.Productswereseparatedona2%agarosegelandstainedwithSYBR®gold.LaneM,100bpladder;lanes1and2,QuickExtract™RNAExtractionKit;lanes3and4,kitfromVendor1;lanes5and6,kitfromVendor2. |
