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Lucigen/AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit/ASB71110/10 Reactions

价格
¥8040.00
货号:ASB71110
浏览量:127
品牌:Lucigen
服务
全国联保
正品保证
正规发票
签订合同
商品描述
  • Formerly from EpicentreProducemicrogramsofbiotin-RNAorbiotin-aRNA
  • Savetimewith30minutehigh-yieldprotocol,orextendtheincubationtoincreaseyieldevenfurther

Applications

  • ProductionofnonrADIoactiveRNAprobesforanypurpose,includingmicroarray(transcriptionprofiling)studies,insituhybridization,andblottingexperiments.

TheAmpliScribe™T7-Flash™Biotin-RNATranscriptionKitenableshigh-yieldsynthesisofbiotin-RNAorbiotin-antisenseRNA(aRNA,alsocalledcRNA).ThekitincorporatesBiotin-16-UTPdirectlyintotheRNAtranscript.Highyieldsofbiotin-RNAareproducedfromaslittleas50ngofinputDNAtemplate.

Features

  • Exceptionallyhighyields.ThekitusesEpicentre'sAmpliScribeT7-Flashrapid,high-yieldinvitrotranscriptiontechnologytogeneratethehighestpossIBLeyieldofbiotin-RNA.
  • Optimized.Invitrotranscriptionreactionconditionsmaximizeyieldofuniformlylabeledbiotin-RNAforhighsignalintensity.
  • Convenient.RnaseInhibitorincluded.

IncubationTime(minutes)

30

60(1hour)

120(2hours)

480(4hours)

TemplateDNA

50ng

5-10µg

15-25µg

30-40µg

60-70µg

100ng

15-20µg

35-45µg

70-80µg

130-140µg

500ng

60-70µg

140-150µg

>180µg

>180µg

1.0µg

125-135µg

>180µg

>180µg

>180µg

Table1.YieldofBiotin-RNA(inmg)fromvaryingamountsofcontroltemplateDNAfromastandard37°C,20mlAmpliScribeT7-FlashBiotin-RNATranscriptionKitreactionovertime.Resultsmayvarydependingonthetemplateused.


ORDERINFORMATION

Contents:AmpliScribe™T7-Flash™EnzymeSolution,RiboGuard™RNaseInhibitor,AmpliScribe™T7-Flash™10XReactionBuffer,NTP/Biotin-UTPPreMix(ATP,CTP,GTP,UTP,andBiotin-16-UTP)Solution,RNase-FreeWater,RNase-FreeDNaseI,DTT,ControlDNATemplate(linearized). Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限,在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应,您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量,可靠性和稳定的性能。