- Producemicrogramsofbiotin-RNAorbiotin-aRNA
- Savetimewith30minutehigh-yieldprotocol,orextendtheincubationtoincreaseyieldevenfurther
Applications
- ProductionofnonrADIoactiveRNAprobesforanypurpose,includingmicroarray(transcriptionprofiling)studies,insituhybridization,andblottingexperiments.
TheAmpliScribe™T7-Flash™Biotin-RNATranscriptionKitenableshigh-yieldsynthesisofbiotin-RNAorbiotin-antisenseRNA(aRNA,alsocalledcRNA).ThekitincorporatesBiotin-16-UTPdirectlyintotheRNAtranscript.Highyieldsofbiotin-RNAareproducedfromaslittleas50ngofinputDNAtemplate.
Features
- Exceptionallyhighyields.ThekitusesEpicentre'sAmpliScribeT7-Flashrapid,high-yieldinvitrotranscriptiontechnologytogeneratethehighestpossIBLeyieldofbiotin-RNA.
- Optimized.Invitrotranscriptionreactionconditionsmaximizeyieldofuniformlylabeledbiotin-RNAforhighsignalintensity.
- Convenient.RnaseInhibitorincluded.
| Table1.YieldofBiotin-RNA(inmg)fromvaryingamountsofcontroltemplateDNAfromastandard37°C,20mlAmpliScribeT7-FlashBiotin-RNATranscriptionKitreactionovertime.Resultsmayvarydependingonthetemplateused. |