T4DNALigasecatalyzestheformationofaphosphodiesterbondbetweentheterminal5′phosphateand3′hydroxylgroupsofduplexDNAorRNA.TheenzymeefficientlyjoinsbluntandcohesiveendsandrepairssinglestrandednicksinduplexDNA,RNAorDNA/RNAhybrids.

T4DNALigaseVersion	Sizes	LigaseConcentration	ProvidedBuffer
LowConcentrationLigase	1,500U 7,500U	2U/µL	10XT4DNALigaseBuffer
HighConcentrationRapidKit	1,500U 7,500U	10U/µL	2XRapidLigationBuffer, 10XT4DNALigaseBuffer

10XT4DNALigaseBufferiscomposedof500mMTris-HCI,100mMMgCl₂,50mMdithiothreitol,10mMATP,pH7.6@25°C.

2XRapidLigationBufferiscomposedof132mMTris-HCI,20mMMgCl₂,2mMdithiothreitol,2mMATP,15%PEG,pH7.6@25°C.

 

 

UnitDefinition:OneWeissUnitisdefinedastheamountofenzymerequiredtoconvert1nmolof³²P-labeledinorganicpyrophosphateintoNoritadsorbablematerialin20minutesat37°C,usingspecifiedreactionconditions.Note:1WeissUnitisapproximately67cohesiveendunits.

Source:ArecombinantE.colistraincarryingtheclonedT4DNALigasegene.

ORDERINFORMATION

Storagebuffer:T4DNALigaseissuppliedin10mMTris-HCl,50mMKCl,1mMdithiothreitol,0.1mMEDTA,0.1%TritonX-100,50%glycerol,pH7.5@25°C.

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