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蚂蚁淘在线 / 品牌中心 / Lucigen / Lucigen/CircLigase™ ssDNA Ligase/CL4115K/5,000 Units

Lucigen/CircLigase™ ssDNA Ligase/CL4115K/5,000 Units

价格
￥16640.00
货号：CL4115K
浏览量：127
品牌：Lucigen
服务
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商品描述

Thermostableligasethatcatalyzestheintramolecularligation(i.e.circularization)ofssDNAtemplates

  • Epicentre ProductIntramolecularligationofsingle-strandedDNAendscontaining5'-phosphatesand3'-hydroxylgroups
  • ATP-dependent,catalyticenzyme
  • CircularizesssDNA>30baseslong
  • CompatIBLewithhightemperatureligationreactionsduetothermostABIlityofCircLigase™enzyme

Applications

  • ProductionofssDNAtemplatesforrolling-circlereplicationorrolling-circletranscriptionexperiments.
  • ProductionofssDNAtemplatesforRNApolymeraseandRNApolymeraseinhibitorassays.

CircLigase™ssDNALigase*isathermostableATP-dependentligasethatcatalyzesintramolecularligation(i.e.circularization)ofssDNAtemplateshavinga5´-phosphateanda3´-hydroxylgroup.IncontrasttoT4DNALigaseandAmpligase®DNALigase,whichligateDNAendsthatareannealedadjacenttoeachotheronacomplementaryDNAsequence,CircLigasessDNALigaseligatesendsofssDNAintheabsenceofacomplementarysequence.TheenzymeisthereforeusefulformakingcircularssDNAmoleculesfromlinearssDNA.CircularssDNAmoleculescanbeusedassubstratesforrolling-circlereplicationorrolling-circletranscription.

LinearssDNAof>30basesiscircularizedbyCircLigaseenzyme.Understandardreactionconditions,virtuallynolinearconcatamersorcircularconcatamersareproduced.InadditiontoitsactivityonssDNA,CircLigaseenzymealsohasactivityinligatingasingle-strandednucleicacidhavinga3´-hydroxylribonucleotideanda5´-phosphorylatedribonucleotideordeoxyribonucleotide.

UnitDefinition:OneunitofCircLigaseenzymeconverts1pmolofalinear5´-phosphorylatedCircLigaseStandard55-merOligointoanexonucleaseI-resistantcircularformin1hourat60°Cunderstandardassayconditions.

StorageBuffer:50%glycerolcontaining50mMTris-HCl(pH7.5),100mMNaCl,0.1mMEDTA,1mMDTT,and0.1%Triton®X-100.

10XReactionBuffer:0.5MMOPS(pH7.5),0.1MKCl,50mMMgCl2,and10mMDTT.TheReactionBufferdoesnotcontain
ATPorMnCl2whichmustbeaddedtothereactionatfinalconcentrationsof0.05mMATPand2.5mMMnCl2.A2.5-mMsolutionofATPanda50-mMsolutionofMnCl2areincluded.

QualityControl:CircLigasessDNALigaseisfreeofdetectablephosphatase,DNAexo-andendonuclease,andRNaseactivities.

ProductCitations

  1. Polidoros,A.N.etal.(2006)BioTechniques41,35.
  2. Shroff,H.etal.(2005)NanoLetters5(7),1509.
  3. Lin,C.etal.(2006)AngewandteChemie118,7699.
  4. Korlach,J.etal.(2008)Proc.Natl.Acad.Sci.USA105,1176.
  5. McArthur,M.andBibb,M.J.(2008)Proc.Natl.Acad.Sci.USA105,1020.
  6. Shroff,H.etal.(2008)BiophysicalJournal94,2179.
  7. Kuhn,H.andFrank-Kamenetskii,M.D.(2008)NucleicAcidsRes.36,e40.
  8. Murakami,T.et.al.(2008)NucleicAcidsRes.Doi:10.1093/nar/gkn1014

ORDERINFORMATION

CircLigase™ssDNALigaseisprovidedat100U/μl.

Contents:CircLigase™ssDNALigase,CircLigase™10XReactionBuffer,1mMATP,50mMMnCl2,CircLigase™ssDNAControlOligo,SterileWater.

Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限，在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应，您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量，可靠性和稳定的性能。