快报
欢迎光临蚂蚁淘在线！ 请登录 免费注册
商品
品牌
文章
热门搜索：
快报
品牌首页 品牌新闻 品牌简介 产品中心 品牌咨询 联系我们
品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / Lucigen / Lucigen/CircLigase™ ssDNA Ligase/CL4111K/1,000 Units

Lucigen/CircLigase™ ssDNA Ligase/CL4111K/1,000 Units

价格
￥3980.00
货号：CL4111K
浏览量：127
品牌：Lucigen
服务
全国联保
正品保证
正规发票
签订合同
商品描述

Thermostableligasethatcatalyzestheintramolecularligation(i.e.circularization)ofssDNAtemplates

  • Epicentre ProductIntramolecularligationofsingle-strandedDNAendscontaining5'-phosphatesand3'-hydroxylgroups
  • ATP-dependent,catalyticenzyme
  • CircularizesssDNA>30baseslong
  • CompatIBLewithhightemperatureligationreactionsduetothermostABIlityofCircLigase™enzyme

Applications

  • ProductionofssDNAtemplatesforrolling-circlereplicationorrolling-circletranscriptionexperiments.
  • ProductionofssDNAtemplatesforRNApolymeraseandRNApolymeraseinhibitorassays.

CircLigase™ssDNALigase*isathermostableATP-dependentligasethatcatalyzesintramolecularligation(i.e.circularization)ofssDNAtemplateshavinga5´-phosphateanda3´-hydroxylgroup.IncontrasttoT4DNALigaseandAmpligase®DNALigase,whichligateDNAendsthatareannealedadjacenttoeachotheronacomplementaryDNAsequence,CircLigasessDNALigaseligatesendsofssDNAintheabsenceofacomplementarysequence.TheenzymeisthereforeusefulformakingcircularssDNAmoleculesfromlinearssDNA.CircularssDNAmoleculescanbeusedassubstratesforrolling-circlereplicationorrolling-circletranscription.

LinearssDNAof>30basesiscircularizedbyCircLigaseenzyme.Understandardreactionconditions,virtuallynolinearconcatamersorcircularconcatamersareproduced.InadditiontoitsactivityonssDNA,CircLigaseenzymealsohasactivityinligatingasingle-strandednucleicacidhavinga3´-hydroxylribonucleotideanda5´-phosphorylatedribonucleotideordeoxyribonucleotide.

UnitDefinition:OneunitofCircLigaseenzymeconverts1pmolofalinear5´-phosphorylatedCircLigaseStandard55-merOligointoanexonucleaseI-resistantcircularformin1hourat60°Cunderstandardassayconditions.

StorageBuffer:50%glycerolcontaining50mMTris-HCl(pH7.5),100mMNaCl,0.1mMEDTA,1mMDTT,and0.1%Triton®X-100.

10XReactionBuffer:0.5MMOPS(pH7.5),0.1MKCl,50mMMgCl2,and10mMDTT.TheReactionBufferdoesnotcontain
ATPorMnCl2whichmustbeaddedtothereactionatfinalconcentrationsof0.05mMATPand2.5mMMnCl2.A2.5-mMsolutionofATPanda50-mMsolutionofMnCl2areincluded.

QualityControl:CircLigasessDNALigaseisfreeofdetectablephosphatase,DNAexo-andendonuclease,andRNaseactivities.

ProductCitations

  1. Polidoros,A.N.etal.(2006)BioTechniques41,35.
  2. Shroff,H.etal.(2005)NanoLetters5(7),1509.
  3. Lin,C.etal.(2006)AngewandteChemie118,7699.
  4. Korlach,J.etal.(2008)Proc.Natl.Acad.Sci.USA105,1176.
  5. McArthur,M.andBibb,M.J.(2008)Proc.Natl.Acad.Sci.USA105,1020.
  6. Shroff,H.etal.(2008)BiophysicalJournal94,2179.
  7. Kuhn,H.andFrank-Kamenetskii,M.D.(2008)NucleicAcidsRes.36,e40.
  8. Murakami,T.et.al.(2008)NucleicAcidsRes.Doi:10.1093/nar/gkn1014

ORDERINFORMATION

CircLigase™ssDNALigaseisprovidedat100U/μl.

Contents:CircLigase™ssDNALigase,CircLigase™10XReactionBuffer,1mMATP,50mMMnCl2,CircLigase™ssDNAControlOligo,SterileWater.

Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限，在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应，您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量，可靠性和稳定的性能。