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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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商品描述
Magnesium-dependentdigestionofsingle-strandedDNAina5´to3´direction
Specific:DigestsssDNAinthe5'to3direction- HeatInactivated:Heatat65°Cfor20minutestokillenzymeactivity
- FlexIBLe:UsetoremoveprimersfromcompletedPCRortodegradesingle-strandedlinearDNAindsDNAandplasmidpreps
RecJExonuclease,derivedfromE.coli,catalyzesdegradationofssDNAina5´to3´direction.ItsactivityisdependentonMg2+.RecJExonucleasecanbeheat-inactivatedbyincubationat65°Cfor20minutes.
Applications
- RemovalofprimersfromcompletedPCR.
- Degradationofsingle-strandedlinearDNAindsDNAandplasmidpreps.
UnitDefinition:OneunitofRecJExonucleasecatalyzesthereleaseof1nmolofacid-solublenucleotidesfromactivatedsingle-strandedcalfthymusDNAin30minutesat37°Cunderstandardassayconditions.
StorageBuffer:50%glycerolcontaining50mMTris-HCl(pH7.5),0.1mMEDTA,1mMDTT,0.1MNaCl,and0.1%Triton®X-100.
10XReactionBuffer:330mMTris-acetate(pH7.5),660mMpotassiumacetate,100mMmagnesium,acetate,and5.0mMDTT.
QualityControl:RecJExonucleaseisfreeofdetectableRNaseandcontaminatingDNAendonucleaseactivities.
