- Inducehighlevelproteinexpressionfromlac basedpromoters(T7,T5-lac,tac,TRC,lac)
- TightlyregulateexpressionfromT7promoterswithHI-ControlBL21(DE3)ChemicallyCompetentCells
- ClonetargetsandreducebackgroundproteinexpressionwithHI-Control10GChemicallyCompetentCells
TheHI-Control™strainsarebasedonE.cloni 10G(DH10B)andBL21(DE3)strains.Thesestrainsharboraplasmidbearingaconstitutive lacIrepressorgene,whichprovidestightcontroloverpromoterscontainingthe lacOoperator.
Reduceleakyexpression: The LacIrepressorproteinbindstothe lacOoperator,blockingtranscriptionofdownstreamgenes.IntheabsenceofaninductionagentsuchaslactosesugarorIPTG,targetproteinexpressionfromtheadjacentpromoterisgreatlyreduced.
Obtainhighproteinyields: Both10GandBL21(DE3)-basedstrainsexpresshighlevelsofprotein.ForexpressionfromaT7promoter,selecttheHI-ControlBL21(DE3)strain.Otherlac-basedpromoterslikeT5-lac,tac,trc,andlac,canbeusedineitherHI-Controlstrain.
Achievehighefficiencytransformations: HighlyefficientcompetentcellsgiveexcellentresultsfromsmallamountsofplasmidDNA.
| TightlyControlProteinExpression | ||||
| T7Promoters | Non-T7lacpromoters (T5-lac,tac,trc,lac) | Clonewithlowbackgroundtargetexpression | Transformationefficiencies | |
| HI-Control BL21(DE3) | ≥1×107 cfu/µg | |||
| HI-Control 10G | ≥1×109 cfu/µg | |||
Genotypes:
HI-Control™BL21(DE3):F-ompThsdSB(rB-mB-)galdcm(DE3)Mini-FlacIq1(GentR)
HI-Control™10G:mcrAΔ(mrr-hsdRMS-mcrBC)endA1recA1ɸ80dlacZΔM15ΔlacX74araD139Δ(ara,leu)7697galUgalKrpsL(StrR)nupGλ−tonAMini-FlacIq1(GentR)
