快报
欢迎光临蚂蚁淘在线！ 请登录 免费注册
商品
品牌
文章
热门搜索：
快报
品牌首页 品牌新闻 品牌简介 产品中心 品牌咨询 联系我们
品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / Lucigen / Lucigen/TransforMax™ EPI300™ Electrocompetent E. coli/EC300150/50 X 100 µl

Lucigen/TransforMax™ EPI300™ Electrocompetent E. coli/EC300150/50 X 100 µl

价格
￥27800.00
货号：EC300150
浏览量：127
品牌：Lucigen
服务
全国联保
正品保证
正规发票
签订合同
商品描述

StandardcloningforlargeinsertsandGibsonassemblyelectroporation

  • Formerly from EpicentreClonelargeinsertsandtransformlargeplasmids-uptoatleast145kbplasmidDNA.
  • GeneratecloneswithinducIBLecopynumberusingCopyControl™vectors.
  • Achievehightransformationefficiencies:>1×1010cfu/µgpUC19DNA.
  • IncreaseGibsonAssembly®colonycountswithhigh-efficiencyelectroporation
FreeSampleAvailable

Applications

  • Generationofinduciblecopy-numberclonesusingtheCopyControl™CloningSystem.

TransforMax™EPI300™E.colicellslackthetonAgeneandareengineeredforusewithEpicentre'sCopyControl™CDNA,Gene,andPCRCloningKitandotherCopyControlCloningSystems*thatdonotrequirephageT1-resistantcells.ThecellscontainaninduciblemutanttrfAgenewhosegeneproductisrequiredforinitiationofreplicationfromtheoriVoriginofreplication,suchasthatinCopyControlpCC1™vectors.OnLBchloramphenicolplatesorinLBorSOCmediasupplementedwithchloramphenicol,CopyControlclonesgrowninTransforMaxEPI300E.colireplicateatsingle-copynumberfromtheF-factorrepliconbecauseexpressionofthetrfAgeneisrepressed.AdditionofCopyControlInductionSolutioninducesthecellstoexpressthetrfAgeneproductandinducestheirreplicationathigh-copynumberfromoriV(Fig.1).ReplicationfromoriVresultsinhigheryieldsandhigherpurityofclonedDNA.

Benefits

  • Copy-numberofclonesisundertightcontrolofaninduciblepromoterlinkedtothetrfAgene.
  • Hightransformationefficiencywithclonesofallsizes.
  • lacZΔM15forblue/whitescreeningofrecombinants.
  • Restriction-minus[mcrA,Δ(mrr-hsdRMS-mcrBC)]phenotypeenablesefficientcloningofmethylatedDNA.
  • Endonuclease-minus(endA1),toensurehighyieldsofDNA.
  • Recombination-minus(recA1),forgreaterstABIlityoflargeinsertclones.

Figure1.CopynumberofCopyControl™BACclonescanbeinduced10-to20-foldinTransforMax™EPI300™E.coli.TheyieldofBACDNAfromCopyControlBACclonesof110-145kbincreased>14-foldfollowingadditionofCopyControlInductionSolution.U=uninducedcells;I=inducedcells.

Genotype

F-mcrAΔ(mrr-hsdRMS-mcrBC)Φ80dlacZΔM15ΔlacX74recA1endA1araD139Δ(ara,leu)7697galUgalKλ-rpsL(StrR)nupGtrfAdhfr

TransforMaxEPI300ElectrocompetentE.coli

  • Transformationefficiencyof>1x1010cfu/µgofpUC19.

TransforMaxEPI300ChemicallyCompetentE.coli

  • Transformationefficiencyof>5x108cfu/µgofpUC19.

*Coveredbyissuedand/orpendingpatents.


ORDERINFORMATION

1,000Xconcentratedsolution.Filtersterilized. Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限，在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应，您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量，可靠性和稳定的性能。