MC1061F-ElectrocompetentCells.Anon-ambersuppressorstrain,identicaltoSS320exceptthatitlackstheF′episome.Highlyefficient(≥4×10¹⁰cfu/µg)electrocompetentcellsforgeneralcloningorPhageDisplay.NotethatasanF-strain,itcannotbeusedforre-infectionbyfilamentousphage. Thisstrainnotincludedinthesamplekit.

• TG1ElectrocompetentCells.Anambersuppressorstrain(supE)preparedashighlyefficient(≥4×10¹⁰cfu/µg)electrocompetentcellsforphagedisplaylibraryscreening.Thesecellsareusedforphagedisplayandproteinexpression.


• SS320ElectrocompetentCells.Anon-ambersuppressorstrain(sometimescalledMC1061F')preparedashighlyefficient(≥4×10¹⁰cfu/µg)electrocompetentcellsforphagedisplaylibraryscreening.Thesecellshavethehighestavailabletransformationefficiencyofanystrainusedforphagedisplay.
• ER2738ElectrocompetentCells.Anambersuppressorstrain(glnV)preparedashighlyefficient(≥2×10¹⁰cfu/µg)electrocompetentcellsforphagedisplaylibraryscreening.ThisstrainisrecommendedforusewithNewEnglandBiolab'sPh.D.™PhageDisplayKits.

Table1.CompetentCellsforPhageDisplaylibraries

CustomCompetentCells

BulkLucigencellsoryourstrainofE.coli,madetothehighestpossIBLetransfectionefficiencywithcustomdispensing!SeeCustomCompetentCellsfordetailedinformation.

ORDERINFORMATION

CompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedasDUOs(2transformationspertube).RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise.

PLEASENOTE:Bulkquantities(10timeslargerthanthelargestretailpackagesizebelow)and/orcustompackagingareavailableatveryattractivepricesforallLucigenCompetentCells.PleasecontactLucigen.

Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限，在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应，您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量，可靠性和稳定的性能。

CellLines	TransformationEfficiency (cfu/µgpUCDNA)	CloningMethylatedDNA	BAC,CosmidCloning	Blue/WhiteScreening
TG1Electrocompetent	>4×1010	YES	NO	YES IPTGinductionrequired
SS320(MC1061F')Electrocompetent	>4×1010	YES	NO	YES IPTGinductionrequired
MC1061F-Electrocompetent	>4×1010	YES	NO	YES IPTGinductionrequired
ER2738Electrocompetent	>2×1010	YES	NO	YES IPTGinductionrequired