- Perfectforantibodyphagedisplayorpeptidephagedisplaylibrarycreation
- ThehighestefficiencyTG1competentcellsavailable:≥4×1010cfu/µg.
- YouronlysourceforelectrocompetentSS320andER2738cells
- Ambersuppressorandnon-ambersuppressorlinesformaximumflexibility
- HighefficiencyMC1061F-cellsforcloningorcontrolreactions
- Createlargerlibraries;speeddiscovery
- Bulkcustomdispensingavailablewithallofourphagedisplaycells
FreeSampleAvailable
MC1061F-ElectrocompetentCells.Anon-ambersuppressorstrain,identicaltoSS320exceptthatitlackstheF′episome.Highlyefficient(≥4×1010cfu/µg)electrocompetentcellsforgeneralcloningorPhageDisplay.NotethatasanF-strain,itcannotbeusedforre-infectionbyfilamentousphage. Thisstrainnotincludedinthesamplekit.
- TG1ElectrocompetentCells.Anambersuppressorstrain(supE)preparedashighlyefficient(≥4×1010cfu/µg)electrocompetentcellsforphagedisplaylibraryscreening.Thesecellsareusedforphagedisplayandproteinexpression.
- SS320ElectrocompetentCells.Anon-ambersuppressorstrain(sometimescalledMC1061F')preparedashighlyefficient(≥4×1010cfu/µg)electrocompetentcellsforphagedisplaylibraryscreening.Thesecellshavethehighestavailabletransformationefficiencyofanystrainusedforphagedisplay.
- ER2738ElectrocompetentCells.Anambersuppressorstrain(glnV)preparedashighlyefficient(≥2×1010cfu/µg)electrocompetentcellsforphagedisplaylibraryscreening.ThisstrainisrecommendedforusewithNewEnglandBiolab'sPh.D.™PhageDisplayKits.
Figure1.TransformationefficiencyofLucigen'selectrocompetentbacterialcellsforphagedisplaycomparedtocompetitor'sspecification. |
Table1.CompetentCellsforPhageDisplaylibraries
CellLines | TransformationEfficiency | CloningMethylatedDNA | BAC,CosmidCloning | Blue/WhiteScreening |
TG1Electrocompetent | >4×1010 | YES | NO | YES |
SS320(MC1061F')Electrocompetent | >4×1010 | YES | NO | YES |
MC1061F-Electrocompetent | >4×1010 | YES | NO | YES |
ER2738Electrocompetent | >2×1010 | YES | NO | YES |